Human FGF basic/FGF2/bFGF Antibody

Catalog #: MAB2331 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB2331-100
MAB2331-SP

Human FGF basic/FGF2/bFGF ELISA Standard Curve.

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All FGF basic/FGF2/bFGF Antibodies

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Human FGF basic/FGF2/bFGF Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF basic/FGF2/bFGF in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 10043R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human FGF basic/FGF2/bFGF
Accession # P09038

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB233R).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FGF basic/FGF2/bFGF DuoSet ELISA Kit (Catalog # DY233) for convenient development of a sandwich ELISA or the Human FGF basic/FGF2/bFGF Quantikine ELISA Kit (Catalog # DFB50) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human FGF basic/FGF2/bFGF ELISA Standard Curve. Recombinant Human FGF basic/FGF2/bFGF protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody(Catalog # MAB233R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2331) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF-2, is a member of the FGF family of growth factors.

Long Name

Fibroblast Growth Factor basic

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Alternate Names

basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin