Human FAP QuicKit ELISA Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)
Assay Range
54.7 - 3,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human FAP.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine QuicKit Human FAP Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human FAP levels in cell culture supernates, serum, and plasma. It contains recombinant human FAP and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FAP showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural FAP.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
1 |
2 |
| n |
20 |
20 |
10 |
10 |
| Mean (pg/mL) |
283 |
1511 |
315 |
1835 |
| Standard Deviation |
14.1 |
103 |
34.4 |
84.2 |
| CV% |
5 |
6.8 |
10.9 |
4.6 |
Recovery
The recovery of human FAP spiked to three different levels in samples throughout the range of the assay in cell culture media was evaluated.
| Sample Type |
Average % Recovery |
Range % |
| Cell Culture Media (n=4) |
102 |
94-108 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FAP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Human FAP Quantikine QuicKit ELISA Standard Curve
Product Datasheets
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Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Fibroblast Activation Protein alpha/FAP
FAP (fibroblast activation protein-alpha; also known as seprase) is a type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue, and may play roles in tumor invasion, tissue remodeling, and wound repair.
Entrez Gene IDs:
2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)
Alternate Names:
170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin
