Human F-box protein 15/FBXO15 Antibody

Catalog #: MAB6035 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB6035
MAB6035-SP

F‑box protein 15/FBXO15 in BG01V Human Embryonic Stem Cells.

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All F-box protein 15/FBXO15 Antibodies

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Human F-box protein 15/FBXO15 Antibody Summary

Species Reactivity

Human

Specificity

Detects human F‑box protein 15/FBXO15 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse F-box protein 15 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 612834

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human F‑box protein 15/FBXO15 isoform 2

Asn298-Tyr434
Accession # Q8NCQ5-2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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F‑box protein 15/FBXO15 in BG01V Human Embryonic Stem Cells. F-box protein 15/FBXO15 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human F-box protein 15/FBXO15 Monoclonal Antibody (Catalog # MAB6035) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Oct-3/4 was detected in cell nuclei using Goat Anti-Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) and stained using NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Specific staining of F-box protein 15 was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry

Detection of FBXO15 antibody in BG01V Human Stem Cells antibody by Flow Cytometry.

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Detection of FBXO15 in BG01V Human Stem Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human F-box protein 15/FBXO15 Monoclonal Antibody (Catalog # MAB6035, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: F-box protein 15/FBXO15

FBXO15 (F-box only protein 15; also FBX15) is a 50‑60 kDa member of the F-box family, O-subfamily of molecules that is most closely related to FBXO8 and O11. It is found in pluripotent stem cells and testis tissue, and apparently serves as a substrate recognition component of the SCF-type E3 ubiquitin ligase. Human FBXO15 (isoform 2 of accession Q8NCQ5) is 434 amino acids (aa) in length. It contains one F-box (aa 1‑41) that likely serves as an interaction domain for SKP1. There are two potential isoforms, one that contains an alternative start site 76 aa upstream of the standard site (isoform 1 of accession Q8NCQ5), and a second that shows complex splicing that includes a 66 aa insertion after Asn35, a Ser substitution for aa 186‑256, and a 106 aa substitution for aa 304‑346. Over aa 298‑434, human FBXO15 shares 53% aa identity with mouse FBXO15.

Entrez Gene IDs

201456 (Human); 361354 (Rat)

Alternate Names

F-box only protein 15; Fbox protein 15; F-box protein 15; FBX15; FBX15MGC39671; FBXO15