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Human ErbB3/Her3 Antibody

Catalog #: MAB348
8 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
MAB348-SP
MAB348-500
MAB348-100
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ErbB3/Her3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ErbB3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with recombinant human (rh) EGF R, rhErbB4, or rhErbB2.
Source
Monoclonal Mouse IgG1 Clone # 66219
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB3
Ser20-Thr643
Accession # P21860
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ErbB3/Her3 Fc Chimera (Catalog # 348-RB)
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human lung, human lung cancer tissue, and human breast

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB3/Her3

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation

References
  1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. 86:9193.
  2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
  3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
  4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
  6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  9. Schlessinger, J. (2000) Cell 103:211.
  10. Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB3
Entrez Gene IDs
2065 (Human); 13867 (Mouse)
Alternate Names
c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

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Citations for Human ErbB3/Her3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Soluble SORLA enhances neurite outgrowth and regeneration through activation of the EGF Receptor/ERK signaling axis
    Authors: J Stupack, XP Xiong, LL Jiang, T Zhang, L Zhou, A Campos, B Ranscht, W Mobley, EB Pasquale, H Xu, TY Huang
    J. Neurosci., 2020-06-29;0(0):.
  2. BACE1 Activity Is Modulated by Cell-Associated Sphingosine-1-Phosphate
    Authors: Nobumasa Takasugi, Tomoki Sasaki, Kunimichi Suzuki, Satoko Osawa, Hayato Isshiki, Yukiko Hori et al.
    The Journal of Neuroscience
  3. SUMO1-conjugation is altered during normal aging but not by increased amyloid burden.
    Authors: Stankova T, Piepkorn L, Bayer TA et al.
    Aging Cell
  4. A large-scale proteomic analysis of human embryonic stem cells.
    Authors: Schulz TC, Swistowska AM, Liu Y
    BMC Genomics, 2007-12-27;8(0):478.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. APP and DYRK1A regulate axonal and synaptic vesicle protein networks and mediate Alzheimer's pathology in trisomy 21 neurons
    Authors: CI Wu, EA Vinton, RV Pearse, K Heo, AJ Aylward, YC Hsieh, Y Bi, S Adeleye, S Fancher, DM Duong, NT Seyfried, TL Schwarz, TL Young-Pear
    Molecular Psychiatry, 2022-02-23;0(0):.
  6. Molecular Differences and Similarities between Alzheimer's Disease and the 5XFAD Transgenic Mouse Model of Amyloidosis
    Authors: Chera L. Maarouf, Tyler A. Kokjohn, Charisse M. Whiteside, MiMi P. Macias, Walter M. Kalback, Marwan N. Sabbagh et al.
    Biochemistry Insights
  7. Small Molecule SARM1 Inhibitors Recapitulate the SARM1-/- Phenotype and Allow Recovery of a Metastable Pool of Axons Fated to Degenerate
    Authors: RO Hughes, T Bosanac, X Mao, TM Engber, A DiAntonio, J Milbrandt, R Devraj, R Krauss
    Cell Reports, 2021-01-05;34(1):108588.
  8. PEDF Expression Affects the Oxidative and Inflammatory State of Choroidal Endothelial Cells
    Authors: M Farnoodian, CM Sorenson, N Sheibani
    Am. J. Physiol., Cell Physiol., 2018-01-10;0(0):.

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Human ErbB3/Her3 Antibody
By Anonymous on 01/24/2022
Application: WB Sample Tested: Lung carcinoma Species: Human
Western Blot Human ErbB3/Her3 Antibody MAB348