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Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) PE-conjugated Antibody

Catalog #: FAB9589P Datasheet
Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
FAB9589P-100
Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry. 
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Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu5
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human ErbB2/Her2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ErbB2/Her2 antibody in MCF-7 human cell line antibody by Flow Cytometry.  View Larger

Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry.  MCF-7 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) PE-conjugated Monoclonal Antibody (Catalog # FAB9589P, filled histogram) or irrelevant antibody (open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB2/Her2

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB1). This antibody can be used in flow cytometry or various assay formats to measure Her2 expression.

Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

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