Human ErbB2/Her2 Quantikine ELISA Kit Summary
Product Summary
Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 471 | 1379 | 2681 | 489 | 1419 | 2667 |
| Standard Deviation | 6.71 | 16.3 | 31.1 | 18.7 | 56.4 | 93.5 |
| CV% | 1.4 | 1.2 | 1.2 | 3.8 | 4 | 3.5 |
Recovery
The recovery of human ErbB2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 90-120 |
| EDTA Plasma (n=4) | 99 | 89-118 |
| Heparin Plasma (n=4) | 104 | 92-119 |
| Serum (n=4) | 99 | 91-115 |
| Urine (n=4) | 101 | 94-106 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: ErbB2/Her2
ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human ErbB2/Her2 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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The unique monoclonal antibodies and immunochemical assay for comprehensive determination of the cell-bound and soluble HER2 in different biological samples
Authors: Antos, A;Topolska-Wo?, A;Wo?, M;Mitura, A;Sarzy?ska, P;Lipi?ski, T;Kurylcio, A;Zió?kowski, P;?witalska, M;Tkaczuk-W?ach, J;Gamian, A;Polkowski, WP;Staniszewska, M;
Scientific reports
Species: Human
Sample Types: Serum
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ELISA assay employing epitope-specific monoclonal antibodies to quantify circulating HER2 with potential application in monitoring cancer�patients undergoing therapy with trastuzumab
Authors: V Agnolon, A Contato, A Meneghello, E Tagliabue, G Toffoli, M Gion, F Polo, ASC Fabricio
Sci Rep, 2020-02-20;10(1):3016.
Species: Human
Sample Types: Serum
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