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Human ErbB2/Her2 APC-conjugated Antibody

Catalog #: FAB1129A
5 Citations Datasheet
Catalog # Availability Size / Price Qty
FAB1129A
Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry.
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Citations (5)
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Human ErbB2/Her2 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ErbB2/Her2 in ELISAs and Western blots. In ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4.
Source
Monoclonal Mouse IgG2B Clone # 191924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2
Thr23-Thr652
Accession # P04626
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ErbB2/Her2 antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 APC-conjugated Monoclonal Antibody (Catalog # FAB1129A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (Human Epidermal Growth Factor Receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the Epidermal Growth Factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-Kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-Catenin and Plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).

References
  1. Coussens, L. et. al. (1985) Science 230:1132.
  2. Yamamoto, T. et. al. (1986) Nature 319:230.
  3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et. al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

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Citations for Human ErbB2/Her2 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer
    Authors: Y Wang, L Guo, L Feng, W Zhang, T Xiao, X Di, G Chen, K Zhang
    Oncol. Rep., 2018-03-20;39(5):2147-2159.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. ERBB signaling attenuates proinflammatory activation of nonclassical monocytes
    Authors: Sergey Ryzhov, Anton Matafonov, Cristi L. Galindo, Qinkun Zhang, Truc-Linh Tran, Daniel J. Lenihan et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  3. Asymmetric segregation of template DNA strands in basal-like human breast cancer cell lines.
    Authors: Liu W, Jeganathan G, Amiri S, Morgan K, Ryan B, Pine S
    Mol Cancer, 2013-11-15;12(1):139.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Synergistic Chemoimmunotherapy of Epithelial Ovarian Cancer Using ErbB-Retargeted T Cells Combined with Carboplatin
    Authors: Ana C. Parente-Pereira, Lynsey M. Whilding, Nancy Brewig, Sjoukje J. C. van der Stegen, David M. Davies, Scott Wilkie et al.
    The Journal of Immunology
  5. A Designed Small Molecule Inhibitor of a Non-Coding RNA Sensitizes HER2 Negative Cancers to Herceptin
    Authors: MG Costales, DG Hoch, D Abegg, JL Childs-Dis, SP Velagapudi, A Adibekian, MD Disney
    J. Am. Chem. Soc., 2019-02-06;0(0):.

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