Human/Equine Cytochrome c Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Immunoprecipitation of Human/Mouse Cytochrome c. Cytochrome c was immunoprecipitated from lysates (5 x 106cells) of Jurkat human acute T cell leukemia and CTLL-2 mouse cytotoxic T cell line following incubation with 5 µg Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) for 1 hour at 4 ºC. Cytochrome c-antibody complex-es were absorbed using Protein A Immunoprecipitin (Life Tech-nologies). Immunoprecipitated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation.
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Cytochrome c in Human PBMCs. Cytochrome c was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA using Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytochrome c
Cytochrome c is a mitochondrial electron transport protein that functions in oxidative phosphorylation. Release of Cytochrome c is an early step in apoptotic cell death. Human and equine Cytochrome c share 89% amino acid sequence identity.
Product Datasheets
Citation for Human/Equine Cytochrome c Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Ellipticine derivative NSC 338258 represents a potential new antineoplastic agent for the treatment of multiple myeloma.
Authors: Tian E, Landowski TH, Stephens OW, Yaccoby S, Barlogie B, Shaughnessy JD
Mol. Cancer Ther., 2008-03-04;7(3):500-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC
FAQs
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