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Human EphA1 Antibody

Catalog #: AF638
4 Citations Datasheet
Catalog # Availability Size / Price Qty
AF638
AF638-SP
Detection of Human EphA1 by Western Blot.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EphA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EphA1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) EphA3, rmEphA8, rmEphA4, recombinant rat (rr) EphA5, rmEphA2, rmEphA6, and rmEphA7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EphA1
Lys26-Glu547
Accession # AAD43440
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
MCF-7 human breast cancer cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EphA1 antibody by Western Blot. View Larger

Detection of Human EphA1 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EphA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EphA1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EphA1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

EphA1 in MCF‑7 Human Cell Line. EphA1 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human EphA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF638) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA1

EphA1, also known as Eph and Esk (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA1 has been shown to bind ephrin-A1 (2, 3). The extracellular domains of mouse and human EphA1 share greater than 91% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Entrez Gene IDs
2041 (Human); 13835 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; EPH receptor A1; eph tyrosine kinase 1; EphA1; EPHephrin type-A receptor 1; EPHT; EPHT1erythropoietin-producing hepatoma amplified sequence; Esk; MGC163163; oncogene EPH; soluble EPHA1 variant 1; soluble EPHA1 variant 2; Tyrosine-protein kinase receptor EPH

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Citations for Human EphA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Matrix Rigidity Controls Epithelial-Mesenchymal Plasticity and Tumor Metastasis via a Mechanoresponsive EPHA2/LYN Complex
    Authors: Laurent Fattet, Hae-Yun Jung, Mike W. Matsumoto, Brandon E. Aubol, Aditya Kumar, Joseph A. Adams et al.
    Developmental Cell
  2. Alteration of the EphA2/Ephrin-A signaling axis in psoriatic epidermis.
    Authors: Gordon K, Kochkodan J, Blatt H, Lin S, Kaplan N, Johnston A, Swindell W, Hoover P, Schlosser B, Elder J, Gudjonsson J, Getsios S
    J Invest Dermatol, 2012-11-29;133(3):712-22.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. EphA2/Ephrin-A1 Signaling Complexes Restrict Corneal Epithelial Cell Migration
    Authors: Nihal Kaplan, Anees Fatima, Han Peng, Paul J. Bryar, Robert M. Lavker, Spiro Getsios
    Investigative Opthalmology & Visual Science
  4. Ligand Targeting of EphA2 Enhances Keratinocyte Adhesion and Differentiation via Desmoglein 1
    Authors: Samantha Lin, Kristin Gordon, Nihal Kaplan, Spiro Getsios
    Molecular Biology of the Cell

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