Human EpCAM/TROP-1 Fluorescein-conjugated Antibody Summary
Extracellular domain
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of EpCAM/TROP‑1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human EpCAM/TROP-1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB9601F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EpCAM/TROP1
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).
- Balzar, M. et al. (1999) J. Mol. Med. 77:699.
- Boer, C.J, et al. (1999) J. Pathol. 188:201.
- Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
- Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
- Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Product Datasheets
Citations for Human EpCAM/TROP-1 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Clinical molecular subtyping reveals intrinsic mesenchymal reprogramming in gastric cancer cells
Authors: Jang, E;Shin, MK;Kim, H;Lim, JY;Lee, JE;Park, J;Kim, J;Kim, H;Shin, Y;Son, HY;Choi, YY;Hyung, WJ;Noh, SH;Suh, JS;Sung, JY;Huh, YM;Cheong, JH;
Experimental & molecular medicine
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
An Automatic Platform Based on Nanostructured Microfluidic Chip for Isolating and Identification of Circulating Tumor Cells
Authors: HJ Jou, LY Chou, WC Chang, HC Ho, WT Zhang, PY Ling, KH Tsai, SH Chen, TH Chen, PH Lo, M Chen, HT Hsu
Micromachines, 2021-04-21;12(5):.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Detection -
The Effects of Lentivirus-Mediated Gene Silencing of RAR&beta on the Stemness Capability of Non-Small Cell Lung Cancer
Authors: NH Abu Halim, N Zakaria, K Theva Das, J Lin, MN Lim, KS Fakiruddin, BH Yahaya
Journal of Cancer, 2021-04-19;12(12):3468-3485.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
PARP inhibition sensitizes colorectal cancer-initiating cells to chemotherapy
Authors: A Jarrar, F Lotti, J DeVecchio, S Ferrandon, G Gantt, A Mace, G Karagkouni, M Orloff, M Venere, M Hitomi, J Lathia, JN Rich, MF Kalady
Stem Cells, 2018-11-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro.
Authors: Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G
BMC Cancer, 2015-05-07;15(0):372.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N
J Exp Med, 2013-12-09;210(13):2851-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Calcium channel a2 delta 1 is essential for pancreatic tumor-initiating cells through sequential phosphorylation of PKM2
Authors: Liu J, Tao M, Zhao W et al.
Cellular and molecular gastroenterology and hepatology
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