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EpCAM/TROP-1 Antibody

Human
EpCAM/TROP-1 Antibody

Catalog #: AF960
36 Citations Datasheet
Catalog # Availability Size / Price Qty
AF960
AF960-SP
Detection of Human EpCAM/TROP‑1 by Western Blot.
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Citations (36)
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Human
EpCAM/TROP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EpCAM/TROP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TROP-2 and approximately 26% cross-reactivity with recombinant mouse EpCAM/TROP-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1, extracellular domain
Gln24-Lys265
Accession # CAA32870
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL)
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
0.3-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EpCAM/TROP-1 antibody by Western Blot. View Larger

Detection of Human EpCAM/TROP‑1 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, MCF-7 human breast cancer cell line, and BG01V human embryonic stem cells. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for EpCAM/TROP-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of EpCAM/TROP-1 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of EpCAM/TROP‑1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry EpCAM/TROP-1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

EpCAM/TROP‑1 in BG01V Human Embryonic Stem Cells. EpCAM/TROP-1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry EpCAM/TROP-1 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

EpCAM/TROP‑1 in Human Colon. EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry EpCAM/TROP-1 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

EpCAM/TROP‑1 in Human Colon. EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western View Larger

Detection of Human EpCAM/TROP‑1 by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for EpCAM/TROP‑1 at approximately 52 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry View Larger

Detection of EpCAM/TROP‑1 in HT-29 Human Colon Adenocarcinoma Cell Line (Positive) and Jurkat Human Acute T Cell Leukemia Cell Line (Negative) Cells. EpCAM/TROP‑1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and Jurkat human acute T cell leukemia cell line (negative) cells using Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamous epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

References
  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Boer, C.J. et al. (1999) J. Pathol. 188:201.
  3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Long Name
Epithelial Cell Adhesion Molecule
Entrez Gene IDs
4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

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Citations for Human
EpCAM/TROP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Studies with Human-Induced Pluripotent Stem Cells Reveal That CTNS Mutations Can Alter Renal Proximal Tubule Differentiation
    Authors: Thiyagarajan, R;Taub, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Cryptotanshinone is a candidate therapeutic agent for interstitial lung disease associated with a BRICHOS-domain mutation of SFTPC
    Authors: Motoyasu Hosokawa, Ryuta Mikawa, Atsuko Hagiwara, Yukiko Okuno, Tomonari Awaya, Yuki Yamamoto et al.
    iScience
  3. Early-onset tufting enteropathy in HAI-2-deficient mice is independent of matriptase-mediated cleavage of EpCAM
    Authors: Roman Szabo, Makiko Kawaguchi, Hiroaki Kataoka, Thomas H. Bugge
    Development
  4. IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer
    Authors: Zhao, C;Wang, D;Li, Z;Zhang, Z;Xu, Y;Liu, J;Lei, Q;Han, D;Huo, Y;Liu, S;Li, L;Zhang, Y;
    International immunopharmacology
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, ICC
  5. Expression and Therapeutic Targeting of TROP-2 in Treatment-Resistant Prostate Cancer
    Authors: Jamie M. Sperger, Kyle T. Helzer, Charlotte N. Stahlfeld, Dawei Jiang, Anupama Singh, Katherine R. Kaufmann et al.
    Clinical Cancer Research
  6. A distal lung organoid model to study interstitial lung disease, viral infection and human lung development
    Authors: Matkovic Leko, I;Schneider, RT;Thimraj, TA;Schrode, N;Beitler, D;Liu, HY;Beaumont, K;Chen, YW;Snoeck, HW;
    Nature protocols
    Species: Human
    Sample Types: Organoid
    Applications: Immunohistochemistry
  7. Hierarchical integration of DNA nanostructures and NanoGold onto a microchip facilitates covalent chemistry-mediated purification of circulating tumor cells in head and neck squamous cell carcinoma
    Authors: Sun, N;Zhang, C;Wang, J;Yue, X;Kim, HY;Zhang, RY;Liu, H;Widjaja, J;Tang, H;Zhang, TX;Ye, J;Qian, A;Liu, C;Wu, A;Wang, K;Johanis, M;Yang, P;Liu, H;Meng, M;Liang, L;Pei, R;Chai-Ho, W;Zhu, Y;Tseng, HR;
    Nano today
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  8. Generation and expansion of transitional lung organoids from human pluripotent stem cells
    Authors: IM Leko, N Schrode, J Torres, M Pezet, TA Thimraj, KG Beaumont, HW Snoeck
    bioRxiv : the preprint server for biology, 2023-02-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer
    Authors: Minyong Chen, Diego M. Assis, Matthieu Benet, Colleen M. McClung, Elizabeth A. Gordon, Shourjo Ghose et al.
    Communications Biology
  10. Prostate cancer extracellular vesicle digital scoring assay - a rapid noninvasive approach for quantification of disease-relevant mRNAs
    Authors: JJ Wang, N Sun, YT Lee, M Kim, T Vagner, K Rohena-Riv, Z Wang, Z Chen, RY Zhang, J Lee, C Zhang, H Tang, J Widjaja, TX Zhang, D Qi, PC Teng, YJ Jan, KC Hou, C Hamann, HM Sandler, TJ Daskivich, DJ Luthringer, NA Bhowmick, R Pei, S You, DD Vizio, HR Tseng, JF Chen, Y Zhu, EM Posadas
    Nano Today, 2023-01-03;48(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Capture Assay
  11. Reversible epigenetic alterations regulate class I HLA loss in prostate cancer
    Authors: Tamara S. Rodems, Erika Heninger, Charlotte N. Stahlfeld, Cole S. Gilsdorf, Kristin N. Carlson, Madison R. Kircher et al.
    Communications Biology
  12. Integrated Analytical System for Clinical Single‐Cell Analysis
    Authors: Hannah M. Peterson, Lip Ket Chin, Yoshi Iwamoto, Juhyun Oh, Jonathan C. T. Carlson, Hakho Lee et al.
    Advanced Science
  13. Targeted Inhibition of LPL/FABP4/CPT1 fatty acid metabolic axis can effectively prevent the progression of nonalcoholic steatohepatitis to liver cancer
    Authors: H Yang, Q Deng, T Ni, Y Liu, L Lu, H Dai, H Wang, W Yang
    International journal of biological sciences, 2021-10-11;17(15):4207-4222.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  14. Development and initial clinical testing of a multiplexed circulating tumor cell assay in patients with clear cell renal cell carcinoma
    Authors: Rory M. Bade, Jennifer L. Schehr, Hamid Emamekhoo, Benjamin K. Gibbs, Tamara S. Rodems, Matthew C. Mannino et al.
    Molecular Oncology
  15. Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids
    Authors: J Zhang, LTH Nguyen, R Hickey, N Walters, X Wang, KJ Kwak, LJ Lee, AF Palmer, E Reátegui
    Scientific Reports, 2021-04-13;11(1):8034.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  16. Improving hexaminolevulinate enabled cancer cell detection in liquid biopsy immunosensors
    Authors: KM Chan, J Gleadle, J Li, TD Michl, K Vasilev, M MacGregor
    Scientific Reports, 2021-03-31;11(1):7283.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Capture
  17. Detection of Epithelial Cell Adhesion Molecule in Feline Normal and Tumor Cell Lines and Tissues With Selected Commercial Anti-human EpCAM Antibodies
    Authors: Christa Y. Heyward, Lynn Dong, Hayk Shakhzadyan, Christopher Wan, Tracy Stokol
    Frontiers in Veterinary Science
  18. Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells
    Authors: Hiroyuki Koike, Kentaro Iwasawa, Rie Ouchi, Mari Maezawa, Masaki Kimura, Asuka Kodaka et al.
    Nature Protocols
  19. Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
    Authors: Pooja Hor, Justin K. Ichida, Zea Borok, Amy L. Ryan
    STAR Protocols
  20. PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer
    Authors: LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang
    Cell Rep, 2020-10-13;33(2):108253.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  21. Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring
    Authors: N Sun, YT Lee, RY Zhang, R Kao, PC Teng, Y Yang, P Yang, JJ Wang, M Smalley, PJ Chen, M Kim, SJ Chou, L Bao, J Wang, X Zhang, D Qi, J Palomique, N Nissen, SB Han, S Sadeghi, RS Finn, S Saab, RW Busuttil, D Markovic, D Elashoff, HH Yu, H Li, AP Heaney, E Posadas, S You, JD Yang, R Pei, VG Agopian, HR Tseng, Y Zhu
    Nat Commun, 2020-09-07;11(1):4489.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  22. Centrosome amplification is a frequent event in circulating tumor cells from subjects with metastatic breast cancer
    Authors: Ashok Singh, Ryan A. Denu, Serena K. Wolfe, Jamie M. Sperger, Jennifer Schehr, Tessa Witkowsky et al.
    Molecular Oncology
  23. A Tgfbr1/Snai1-dependent developmental module at the core of vertebrate axial elongation
    Authors: André Dias, Anastasiia Lozovska, Filip J Wymeersch, Ana Nóvoa, Anahi Binagui-Casas, Daniel Sobral et al.
    eLife
  24. Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer
    Authors: C Morral, J Stanisavlj, X Hernando-M, E Mereu, A Álvarez-Va, C Cortina, D Stork, F Slebe, G Turon, G Whissell, M Sevillano, A Merlos-Suá, À Casanova-M, C Moutinho, SW Lowe, LE Dow, A Villanueva, E Sancho, H Heyn, E Batlle
    Cell Stem Cell, 2020-05-11;26(6):845-861.e12.
  25. Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
    Authors: S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi
    Sci Rep, 2020-04-06;10(1):5989.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Biliary Atresia Relevant Human Induced Pluripotent Stem Cells Recapitulate Key Disease Features in a Dish
    Authors: Lipeng Tian, Zhaohui Ye, Kim Kafka, Dylan Stewart, Robert Anders, Kathleen B. Schwarz et al.
    Journal of Pediatric Gastroenterology & Nutrition
  27. Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system
    Authors: D Garnier, R Li, F Delbos, A Fourrier, C Collet, C Guguen-Gui, C Chesné, TH Nguyen
    Sci Rep, 2018-05-29;8(1):8222.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. Integration of Magnetic Bead-Based Cell Selection into Complex Isolations
    Authors: Hannah M. Pezzi, David J. Niles, Jennifer L. Schehr, David J. Beebe, Joshua M. Lang
    ACS Omega
  29. Interrogating Bronchoalveolar Lavage Samples via Exclusion-Based Analyte Extraction
    Authors: Jacob J. Tokar, Jay W. Warrick, David J. Guckenberger, Jamie M. Sperger, Joshua M. Lang, J. Scott Ferguson et al.
    SLAS Technology
  30. Fast and selective cell isolation from blood sample by microfiber fabric system with vacuum aspiration
    Sci Technol Adv Mater, 2016-11-25;17(1):807-815.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  31. HER2 and EGFR overexpression support metastatic progression of prostate cancer to bone
    Cancer Res., 2016-10-28;0(0):.
    Species: Human
    Sample Types: Whole Blood
    Applications: CTC
  32. EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells that Promote Epithelial-to-Mesenchymal Transition
    Cancer Res, 2016-08-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  33. Efficient and Controlled Generation of 2D and 3D Bile Duct Tissue from Human Pluripotent Stem Cell-Derived Spheroids
    Authors: Lipeng Tian, Abhijeet Deshmukh, Zhaohui Ye, Yoon-Young Jang
    Stem Cell Reviews and Reports
  34. Fibroblasts Influence Survival and Therapeutic Response in a 3D Co-Culture Model
    Authors: Meher Majety, Leon P. Pradel, Manuela Gies, Carola H. Ries
    PLOS ONE
  35. Stromal gene expression defines poor-prognosis subtypes in colorectal cancer.
    Authors: Calon A, Lonardo E, Berenguer-Llergo A, Espinet E, Hernando-Momblona X, Iglesias M, Sevillano M, Palomo-Ponce S, Tauriello D, Byrom D, Cortina C, Morral C, Barcelo C, Tosi S, Riera A, Attolini C, Rossell D, Sancho E, Batlle E
    Nat Genet, 2015-02-23;47(4):320-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  36. By promoting cell differentiation, miR-100 sensitizes basal-like breast cancer stem cells to hormonal therapy
    Authors: Annalisa Petrelli, Rosachiara Carollo, Marilisa Cargnelutti, Flora Iovino, Maurizio Callari, Daniela Cimino et al.
    Oncotarget

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EpCAM/TROP-1 Antibody

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