Human
EpCAM/TROP-1 Antibody Summary
Gln24-Lys265
Accession # P16422
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of EpCAM/TROP-1 in Human Colon via Multiplex Immunofluorescence staining on COMET™ EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human EpCAM/TROP-1 Monoclonal Antibody (MAB960) at 1µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
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EpCAM/TROP‑1 in HT-29 Human Cell Line. EpCAM/TROP-1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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EpCAM/TROP‑1 in HT-29 Human Cell Line. EpCAM/TROP-1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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EpCAM/TROP‑1 in Human Adenocarcinoma. EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Mouse Anti-Human EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB960) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of EpCAM/TROP‑1 in HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and Daudi Human Burkitt's Lymphoma Cell Line (Negative) Cells. EpCAM/TROP‑1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and Daudi human Burkitt's lymphoma cell line (negative) cells using Mouse Anti-Human EpCAM/TROP‑1 Monoclonal Antibody (Catalog # MAB960) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EpCAM/TROP1
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with Trop-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).
- Balzar, M. et al. (1999) J. Mol. Med. 77:699.
- Boer, C.J. et al. (1999) J. Pathol. 188:201.
- Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
- Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
- Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Product Datasheets
Citations for Human
EpCAM/TROP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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Plasma extracellular vesicles detected by Single Molecule array technology as a liquid biopsy for colorectal cancer
Authors: Ping Wei, Fei Wu, Bin Kang, Xiaohua Sun, Fabienne Heskia, Alexandre Pachot et al.
Journal of Extracellular Vesicles
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Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration
Authors: Zhenyu Cai, Anling Zhang, Swati Choksi, Weihua Li, Tao Li, Xue-Min Zhang et al.
Cell Research
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Expression of EpCAM and Wnt/ beta -catenin in human colon cancer
Authors: F.Q. Zhou, Y.M. Qi, H. Xu, Q.Y. Wang, X.S. Gao, H.G. Guo
Genetics and Molecular Research
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Effect of leaf position and days post-infiltration on transient expression of colorectal cancer vaccine candidate proteins GA733-Fc and GA733-FcK in Nicotiana benthamiana plant
Authors: K Kim, YJ Kang, SR Park, DS Kim, SW Lee, K Ko, D Ponndorf, K Ko
PeerJ, 2021-04-07;9(0):e10851.
Species: Human, Nicotiana benthamiana
Sample Types: Plant Homogenates, Protein
Applications: ELISA Capture, Western Blot -
Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
Authors: MA Witek, RD Aufforth, H Wang, JW Kamande, JM Jackson, SR Pullagurla, ML Hupert, J Usary, WZ Wysham, D Hilliard, S Montgomery, V Bae-Jump, LA Carey, PA Gehrig, MI Milowsky, CM Perou, JT Soper, YE Whang, JJ Yeh, G Martin, SA Soper
NPJ Precis Oncol, 2017-07-25;1(0):.
Species: Human, Xenograft
Sample Types: Whole Cells, Whole Tissue
Applications: Cell Capture, Flow Cytometry, ICC, IHC-P -
Polymeric mechanical amplifiers of immune cytokine-mediated apoptosis
Authors: MJ Mitchell, J Webster, A Chung, PP Guimarães, OF Khan, R Langer
Nat Commun, 2017-03-20;8(0):14179.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells that Promote Epithelial-to-Mesenchymal Transition
Cancer Res, 2016-08-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Adhesion Assay -
Identification of tumorigenic cells and therapeutic targets in pancreatic neuroendocrine tumors
Authors: GW Krampitz, BM George, SB Willingham, JP Volkmer, K Weiskopf, N Jahchan, AM Newman, D Sahoo, AJ Zemek, RL Yanovsky, JK Nguyen, PJ Schnorr, PK Mazur, J Sage, TA Longacre, BC Visser, GA Poultsides, JA Norton, IL Weissman
Proc Natl Acad Sci USA, 2016-03-31;113(16):4464-9.
Species: Human
Sample Types: Whole Cells
Applications: Phagocytosis Assay -
Integrative genomics identifies YY1AP1 as an oncogenic driver in EpCAM(+) AFP(+) hepatocellular carcinoma.
Authors: Zhao X, Parpart S, Takai A, Roessler S, Budhu A, Yu Z, Blank M, Zhang Y, Jia H, Ye Q, Qin L, Tang Z, Thorgeirsson S, Wang X
Oncogene, 2015-01-19;34(39):5095-104.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Detection of brain tumor cells in the peripheral blood by a telomerase promoter-based assay.
Authors: Macarthur K, Kao G, Chandrasekaran S, Alonso-Basanta M, Chapman C, Lustig R, Wileyto E, Hahn S, Dorsey J
Cancer Res, 2014-02-13;74(8):2152-9.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Activation of hepatic stem cell marker EpCAM by Wnt-beta-catenin signaling in hepatocellular carcinoma.
Authors: Yamashita T, Budhu A, Forgues M, Wang XW
Cancer Res., 2007-11-15;67(22):10831-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
GRHL2-miR-200-ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification
Authors: Vin Yee Chung, Tuan Zea Tan, Ming Tan, Meng Kang Wong, Kuee Theng Kuay, Zhe Yang et al.
Scientific Reports
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Multi-layer assembly of cellulose nanofibrils in a microfluidic device for the selective capture and release of viable tumor cells from whole blood
Authors: Tharagan Kumar, Ruben R. G. Soares, Leyla Ali Dholey, Harisha Ramachandraiah, Negar Abbasi Aval, Zenib Aljadi et al.
Nanoscale
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Expression of Colorectal Cancer Antigenic Protein Fused to IgM Fc in Chinese Cabbage (Brassica rapa)
Authors: Ye-Rin Lee, Chae-Yeon Lim, Sohee Lim, Se Ra Park, Jong-Pil Hong, Jinhee Kim et al.
Plants (Basel)
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EpCAM/TROP-1 Antibody
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