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EpCAM/TROP-1 Antibody

Human
EpCAM/TROP-1 Antibody

Catalog #: MAB9601
17 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB9601-SP
MAB9601-100
MAB9601-500
Detection of EpCAM/TROP‑1 in HT‑29 Human Cell Line by Flow Cytometry.
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Citations (17)
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Human
EpCAM/TROP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EpCAM/TROP-1 in ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 158206
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human EpCAM/TROP-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human EpCAM/TROP‑1 Biotinylated Antibody (Catalog # BAF960)

Standard: Recombinant Human EpCAM/TROP1 Fc Chimera Protein, CF (Catalog # 960-EP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of EpCAM/TROP-1 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of EpCAM/TROP‑1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB9601, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -Actinin (5).

References
  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Boer, C.J, et al. (1999) J. Pathol. 188:201.
  3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Long Name
Epithelial Cell Adhesion Molecule
Entrez Gene IDs
4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

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Citations for Human
EpCAM/TROP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Differentiation of immortalized human multi-lineage progenitor to alveolar type 2-like cells: angiotensin-converting enzyme 2 expression and binding of severe acute respiratory syndrome coronavirus 2 spike and spike 1 proteins
    Authors: Collins DP, Osborn MJ, Steer CJ. et al.
    Cytotherapy
  2. In vitro Differentiation of TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells
    Authors: Collins D, Hapke J, Aravalli R, Steer C
    HMER
  3. SERS characterization of colorectal cancer cell surface markers upon anti‐EGFR treatment
    Authors: Nana Lyu, Bernadette Pedersen, Elena Shklovskaya, Helen Rizos, Mark P. Molloy, Yuling Wang
    Exploration (Beijing)
  4. PD-L1 detection on circulating tumor-derived extracellular vesicles (T-EVs) from patients with lung cancer
    Authors: Fei Wu, Yanzi Gu, Bin Kang, Fabienne Heskia, Alexandre Pachot, Marc Bonneville et al.
    Translational Lung Cancer Research
  5. Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells
    Authors: Ralph El Hallal, Nana Lyu, Yuling Wang
    Molecules
  6. Plasma extracellular vesicles detected by Single Molecule array technology as a liquid biopsy for colorectal cancer
    Authors: Ping Wei, Fei Wu, Bin Kang, Xiaohua Sun, Fabienne Heskia, Alexandre Pachot et al.
    Journal of Extracellular Vesicles
  7. Compact and Filter-Free Luminescence Biosensor for Mobile in Vitro Diagnoses
    Authors: Chen-Han Huang, Yong Il Park, Hsing-Ying Lin, Divya Pathania, Ki Soo Park, Maria Avila-Wallace et al.
    ACS Nano
  8. Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
    Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
    PLoS ONE, 2019-05-02;14(5):e0215990.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  9. New Dioxaborolane Chemistry Enables [(18)F]-Positron-Emitting, Fluorescent [(18)F]-Multimodality Biomolecule Generation from the Solid Phase
    Authors: Erik A. Rodriguez, Ye Wang, Jessica L. Crisp, David R. Vera, Roger Y. Tsien, Richard Ting
    Bioconjugate Chemistry
  10. Pilot Application of Magnetic Nanoparticle-Based Biosensor for Necrotizing Enterocolitis
    Authors: Dokyoon Kim, Changlin Fu, Xuefeng B Ling, Zhongkai Hu, Guozhong Tao, Yingzhen Zhao et al.
    Journal of Proteomics & Bioinformatics
  11. Single-cell magnetic imaging using a quantum diamond microscope.
    Authors: Glenn D, Lee K, Park H, Weissleder R, Yacoby A, Lukin M, Lee H, Walsworth R, Connolly C
    Nat Methods, 2015-06-22;12(8):736-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  12. Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro.
    Authors: Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G
    BMC Cancer, 2015-05-07;15(0):372.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development (Capture)
  13. Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone.
    Authors: Im H, Castro C, Shao H, Liong M, Song J, Pathania D, Fexon L, Min C, Avila-Wallace M, Zurkiya O, Rho J, Magaoay B, Tambouret R, Pivovarov M, Weissleder R, Lee H
    Proc Natl Acad Sci U S A, 2015-04-13;112(18):5613-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoassay Development
  14. Isolation of viable cancer cells in antibody-functionalized microfluidic devices
    Authors: Xiangjun Zheng, Linan Jiang, Joyce Schroeder, Alison Stopeck, Yitshak Zohar
    Biomicrofluidics
  15. Cell receptor and surface ligand density effects on dynamic states of adhering circulating tumor cells
    Authors: Xiangjun Zheng, Luthur Siu-Lun Cheung, Joyce A. Schroeder, Linan Jiang, Yitshak Zohar
    Lab on a Chip
  16. A high-performance microsystem for isolating circulating tumor cells
    Authors: Xiangjun Zheng, Luthur Siu-Lun Cheung, Joyce A. Schroeder, Linan Jiang, Yitshak Zohar
    Lab on a Chip
  17. Rapid detection and profiling of cancer cells in fine-needle aspirates.
    Authors: Lee H, Yoon TJ, Figueiredo JL, Swirski FK, Weissleder R
    Proc. Natl. Acad. Sci. U.S.A., 2009-07-20;106(30):12459-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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