Human Endothelin-1 C-Terminus Antibody

Catalog #: MAB34401 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB34401-100
MAB34401-SP

Human Endothelin‑1 ELISA Standard Curve.

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Human Endothelin-1 C-Terminus Antibody Summary

Species Reactivity

Human

Specificity

Detects human Endothelin-1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 2E1

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Endothelin-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Endothelin‑1 C-Terminus Monoclonal Antibody (Catalog # MAB3440).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Endothelin Pan Specific DuoSet ELISA Kit (Catalog # DY1160) for convenient development of a sandwich ELISA or the Endothelin-1 Quantikine ELISA Kit (Catalog # DET100) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Endothelin‑1 ELISA Standard Curve. Recombinant Human Endothelin-1 protein was serially diluted 2-fold and captured by Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB34401) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endothelin-1

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).

Entrez Gene IDs

1906 (Human); 13614 (Mouse); 24323 (Rat)

Alternate Names

ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME

Product Datasheets

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