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Human Endothelin-1 Antibody

Catalog #: MAB34402 Datasheet
Catalog # Availability Size / Price Qty
MAB34402-100
MAB34402-SP
Detection of Endothelin‑1 in Huvec (positive) and Jurkat (negative) cells.
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Human Endothelin-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endothelin-1 in direct ELISA.
Source
Monoclonal Mouse IgG2B Clone # 1065402
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing EDN1. 
Accession # P05305
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
Immersion fixed HUVEC human umbilical vein endothelial cells (positive) and Jurkat human acute T cell leukemia cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Endothelin‑1 in Huvec (positive) and Jurkat (negative) cells. Endothelin‑1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells (positive) and absent in Jurkat human acute T cell leukemia cells (negative) using Mouse Anti-Human Endothelin‑1 Monoclonal Antibody (Catalog # MAB34402) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endothelin-1

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).

Entrez Gene IDs
1906 (Human); 13614 (Mouse); 24323 (Rat)
Alternate Names
ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME

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