Human Endoglin/CD105 PE-conjugated Antibody Summary
Glu26-Gly586
Accession # Q5T9B9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 PE-conjugated Mono-clonal Antibody (Catalog # FAB10971P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Human Endoglin/CD105 by Flow Cytometry Isolation and characterization of amniotic derived mesenchymal stem cells with renal marker expression(A) Light microscopic image of mesenchymal stem cells isolated from amniotic fluid. A subpopulation of these cells (with renal markers) displays a trumpet shaped phenotype (scale bar = 200 μm). (B) hAFSCs were characterized by flow cytometry (positive for CD24, CD117, CD73, HLA-ABC, CD29, and CD105 but not for CD45, or CD34). (C) Confocal imaging of hAFSCs for renal markers SIX2 and PAX2. (D) Expression of Six2 and Pax2 (left panel) in hAFSCs compared to human embryonic kidney (hEK). Agarose gel analysis of PCR fragments (SIX2: 336 bp; PAX2: 65 bp; KSP: 152bp; GAPDH: 189 bp) (E). hAFSCs were differentiated along adipogenic, osteogenic and chondrogenic lineages (left to right). In the adipogenic differentiation the cells formed lipid vesicles stained with oil red O (left) (magnification 100X). Calcium deposits stained with Alizarin confirmed osteogenesis (center). Micromasses stained with alcian blue confirmed chondrogenesis (right) (scale bar = 200 μm). Quantification of chondrogenic and osteogenic differentiation using dye extraction and quantification with spectrophotometry before and after differentiation (left panel). qPCR analysis of chondrogenic markers (Sox9, Col II) and osteogenic markers (ALP, Runx2 and OST) before and after differentiation (right panel). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0136145), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Endoglin/CD105
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).
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Product Datasheets
Citations for Human Endoglin/CD105 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Enzyme-free isolation of mesenchymal stem cells from decidua basalis of the human placenta
Authors: Srishti Dutta Gupta, Ankita Sen, Priyanshu Priyadarshi, Malancha Ta
STAR Protocols
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Intravenous Cardiac Stem Cell-Derived Exosomes Ameliorate Cardiac Dysfunction in Doxorubicin Induced Dilated Cardiomyopathy
Authors: Adam C. Vandergriff, James Bizetto Meira de Andrade, Junnan Tang, M. Taylor Hensley, Jorge A. Piedrahita, Thomas G. Caranasos et al.
Stem Cells International
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Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections
Authors: Anne-Claire Lagrée, Fabienne Fasani, Clotilde Rouxel, Marine Pivet, Marie Pourcelot, Aurore Fablet et al.
International Journal of Molecular Sciences
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Application of Menstrual Blood Derived Stromal (stem) Cells Exert Greater Regenerative Potency Than Fibroblasts/Keratinocytes in Chronic Wounds of Diabetic Mice
Authors: Ebrahim Mirzadegan, Hannaneh Golshahi, Zahra Saffarian, Haleh Edalatkhah, Maryam Darzi, Somayeh Khorasani et al.
Avicenna Journal of Medical Biotechnology
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Stromal Activation by Tumor Cells: An in Vitro Study in Breast Cancer
Authors: Giuseppe Merlino, Patrizia Miodini, Biagio Paolini, Maria Luisa Carcangiu, Massimiliano Gennaro, Matteo Dugo et al.
Microarrays (Basel)
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Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells
Authors: Aseefhali Bankapur, R. Sagar Krishnamurthy, Elsa Zachariah, Chidangil Santhosh, Basavaraj Chougule, Bhavishna Praveen et al.
PLoS ONE
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A robust super-tough biodegradable elastomer engineered by supramolecular ionic interactions
Authors: Hamed Daemi, Sareh Rajabi-Zeleti, Haritz Sardon, Mehdi Barikani, Ali Khademhosseini, Hossein Baharvand
Biomaterials
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Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells
Authors: Jung Won Kang, Hye Cheong Koo, Sun Young Hwang, Sung Keun Kang, Jeong Chan Ra, Moon Han Lee et al.
Journal of Veterinary Science
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Endothelial CD34 expression and regulation of immune cell response in-vitro
Authors: Arakelian, L;Lion, J;Churlaud, G;Bargui, R;Thierry, B;Mutabazi, E;Bruneval, P;Alberdi, AJ;Doliger, C;Veyssiere, M;Larghero, J;Mooney, N;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Transcriptomic Characterization of Genes Regulating the Stemness in Porcine Atrial Cardiomyocytes during Primary In Vitro Culture
Authors: Bryl, R;Nawrocki, MJ;Jopek, K;Kaczmarek, M;Bukowska, D;Antosik, P;Mozdziak, P;Zabel, M;Dzi?giel, P;Kempisty, B;
Genes
Species: Porcine
Sample Types: Whole Cells
Applications: Flow Cytometry -
Heat Shock Protein 27 Is Involved in the Bioactive Glass Induced Osteogenic Response of Human Mesenchymal Stem Cells
Authors: L Hyväri, S Vanhatupa, M Ojansivu, M Kelloniemi, TK Pakarinen, L Hupa, S Miettinen
Cells, 2023-01-05;12(2):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs)
Authors: HA Hamid, R Ramasamy, MK Mustafa, V Hosseinpou, A Miskon
Scientific Reports, 2022-05-26;12(1):8904.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human Wharton's Jelly Mesenchymal Stem Cells Secretome Inhibits Human SARS-CoV-2 and Avian Infectious Bronchitis Coronaviruses
Authors: MAA Hussein, HAM Hussein, AA Thabet, KM Selim, MA Dawood, AM El-Adly, AA Wardany, A Sobhy, S Magdeldin, A Osama, AM Anwar, M Abdel-Waha, H Askar, EK Bakhiet, S Sultan, AA Ezzat, U Abdel Raou, MM Afifi
Cells, 2022-04-21;11(9):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A Comparative Study of Gene Expression in Menstrual Blood-Derived Stromal Cells between Endometriosis and Healthy Women
Authors: SS Sahraei, F Davoodi As, N Kalhor, M Sheykhhasa, H Fazaeli, SS Moud, A Sheikholes
BioMed Research International, 2022-01-11;2022(0):7053521.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The remarkable effect of menstrual blood stem cells seeded on bilayer scaffold composed of amniotic membrane and silk fibroin aiming to promote wound healing in diabetic mice
Authors: E Mirzadegan, H Golshahi, Z Saffarian, M Darzi, S Khorasani, H Edalatkhah, K Salimineja, S Kazemnejad
International immunopharmacology, 2021-12-02;0(0):108404.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation
Authors: J Liu, X Wei, J Hu, X Tan, X Kang, L Gao, N Li, X Shi, M Yuan, W Hu, M Liu
BMC Oral Health, 2021-09-24;21(1):470.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells
Authors: L Hyväri, S Vanhatupa, HT Halonen, M Kääriäinen, S Miettinen
Stem Cells Int, 2020-09-22;2020(0):8853541.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cell adhesion and culture medium dependent changes in the high frequency mechanical vibration induced proliferation, osteogenesis, and intracellular organization of human adipose stem cells
Authors: HT Halonen, TO Ihalainen, L Hyväri, S Miettinen, JAK Hyttinen
J Mech Behav Biomed Mater, 2019-09-05;101(0):103419.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells
Authors: MR Shokri, M Bozorgmehr, A Ghanavatin, R Falak, M Aleahmad, S Kazemnejad, F Shokri, AH Zarnani
Sci Rep, 2019-07-10;9(1):10007.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
LncRNA-TWIST1 Promoted Osteogenic Differentiation Both in PPDLSCs and in HPDLSCs by Inhibiting TWIST1 Expression
Authors: Y Xu, W Qin, D Guo, J Liu, M Zhang, Z Jin
Biomed Res Int, 2019-06-23;2019(0):8735952.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages
Authors: L Hyväri, M Ojansivu, M Juntunen, K Kartasalo, S Miettinen, S Vanhatupa
Stem Cells Int, 2018-09-12;2018(0):2190657.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The effect of S53P4-based borosilicate glasses and glass dissolution products on the osteogenic commitment of human adipose stem cells
Authors: M Ojansivu, A Mishra, S Vanhatupa, M Juntunen, A Larionova, J Massera, S Miettinen
PLoS ONE, 2018-08-28;13(8):e0202740.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Functional Outcome of Human Adipose Stem Cell Injections in Rat Anal Sphincter Acute Injury Model
Authors: K Kuismanen, M Juntunen, N Narra Giri, H Tuominen, H Huhtala, K Nieminen, J Hyttinen, S Miettinen
Stem Cells Transl Med, 2018-01-31;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients
Authors: C Maroun-Eid, A Ortega-Her, J Modrego, M Abad-Cardi, JA García-Don, L Reinares, N Martell-Cl, D Gómez-Garr
PLoS ONE, 2018-01-05;13(1):e0190494.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies
Authors: PC Dinh, J Cores, MT Hensley, AC Vandergrif, J Tang, TA Allen, TG Caranasos, KB Adler, LJ Lobo, K Cheng
Respir. Res., 2017-06-30;18(1):132.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results
Authors: T Thesleff, K Lehtimäki, T Niskakanga, S Huovinen, B Mannerströ, S Miettinen, R Seppänen-K, J Öhman
Stem Cells Transl Med, 2017-05-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions
Authors: M Patrikoski, MHC Lee, L Mäkinen, XM Ang, B Mannerströ, M Raghunath, S Miettinen
Stem Cells Int, 2017-03-30;2017(0):6909163.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
Authors: PL Mok, SN Leow, AE Koh, HH Mohd Nizam, SL Ding, C Luu, R Ruhaslizan, HS Wong, WH Halim, MH Ng, RB Idrus, SR Chowdhury, CM Bastion, SK Subbiah, A Higuchi, AA Alarfaj, KY Then
Int J Mol Sci, 2017-02-08;18(2):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Study of the microRNA expression profile of foreskin derived mesenchymal stromal cells following inflammation priming
Authors: H Fayyad-Kaz, M Fayyad-Kaz, B Badran, D Bron, L Lagneaux, M Najar
J Transl Med, 2017-01-13;15(1):10.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Therapeutic microparticles functionalized with biomimetic cardiac stem cell membranes and secretome
Authors: J Tang, D Shen, TG Caranasos, Z Wang, AC Vandergrif, TA Allen, MT Hensley, PU Dinh, J Cores, TS Li, J Zhang, Q Kan, K Cheng
Nat Commun, 2017-01-03;8(0):13724.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model
Stem Cells Dev, 2016-10-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The differentiation potential of gingival mesenchymal stem cells induced by apical tooth germ cell?conditioned medium
Mol Med Rep, 2016-09-06;14(4):3565-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The effect of pro-inflammatory cytokines on immunophenotype, differentiation capacity and immunomodulatory functions of human mesenchymal stem cells
Authors: Arash Pourgholam
Cytokine, 2016-06-09;85(0):51-60.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Amniotic Fluid Derived Stem Cells with a Renal Progenitor Phenotype Inhibit Interstitial Fibrosis in Renal Ischemia and Reperfusion Injury in Rats.
Authors: Monteiro Carvalho Mori da M, Zia S, Oliveira Arcolino F, Carlon M, Beckmann D, Pippi N, Luhers Graca D, Levtchenko E, Deprest J, Toelen J
PLoS ONE, 2015-08-21;10(8):e0136145.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Adipose stem cells used to reconstruct 13 cases with cranio-maxillofacial hard-tissue defects.
Authors: Sandor G, Numminen J, Wolff J, Thesleff T, Miettinen A, Tuovinen V, Mannerstrom B, Patrikoski M, Seppanen R, Miettinen S, Rautiainen M, Ohman J
Stem Cells Transl Med, 2014-02-20;3(4):530-40.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
PLoS ONE, 2014-01-29;9(1):e84417.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin.
Authors: Tannetta D, Dragovic R, Gardiner C, Redman C, Sargent I
PLoS ONE, 2013-02-20;8(2):e56754.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human dermis harbors distinct mesenchymal stromal cell subsets.
Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
J. Invest. Dermatol., 2011-11-03;132(3):563-74.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TGFbeta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy.
Authors: Wang C, Tang X, Sun X, Miao Z, Lv Y, Yang Y, Zhang H, Zhang P, Liu Y, DU L, Gao Y, Yin M, Ding M, Deng H
Cell Res., 2011-08-23;22(1):194-207.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
2011-05-19;6(5):e19988.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Transplantation of CTLA4Ig gene-transduced adipose tissue-derived mesenchymal stem cells reduces inflammatory immune response and improves Th1/Th2 balance in experimental autoimmune thyroiditis.
Authors: Choi EW, Shin IS, Lee HW, Park SY, Park JH, Nam MH, Kim JS, Woo SK, Yoon EJ, Kang SK, Ra JC, Youn HY, Hong SH
J Gene Med, 2011-01-01;13(1):3-16.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression of an exogenous human Oct-4 promoter identifies tumor-initiating cells in osteosarcoma.
Authors: Levings PP, McGarry SV, Currie TP, Nickerson DM, McClellan S, Ghivizzani SC, Steindler DA, Gibbs CP
Cancer Res., 2009-07-07;69(14):5648-55.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Isolation and Establishment of Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord
Authors: Umesh Goyal, Chitra Jaiswal, Malancha TA
BIO-PROTOCOL
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