Human Endoglin/CD105 Biotinylated Antibody Summary
Glu26-Gly256
Accession # Q5T9B9
Applications
Human Endoglin/CD105 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Endoglin/CD105 in U937 Human Cell Line. Endoglin/CD105 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Endoglin/CD105 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Endoglin/CD105 in Human Prostate. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Endoglin/CD105 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1097) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Endoglin/CD105 in Human Prostate. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human Endoglin/CD105 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1097) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Mouse Endoglin/CD105 by Western Blot Monocyte specific knock-out of endoglin does not recapitulate the Eng+/- phenotype.(A) Western blot analysis of endoglin protein expression in LysM-Cre-Eng+/+, LysM-Cre-Engfl/+ and LysM-Cre-Engfl/fl cultured macrophages. A representative experiment is shown. (B) Quantification of the Western blots for endoglin protein in LysM-Cre-Eng+/+, LysM-Cre-Engfl/+ and LysM-Cre-Engfl/fl cultured macrophages in two independent experiments(macrophage cultures from 3 individual mice of each genotype were pooled per western blot). (C) Kaplan-Meier survival curve of wild type (WT), LysM-Cre-Engfl/+ and LysM-Cre-Engfl/fl mice 28 days post-MI (n = 6–10). (D) Cardiac function in percentage ejection fraction (%EF) 14 days post-MI. Cardiac function was measured by ultrasound in long axis view (n = 5–9). Data are shown as mean ± SEM, *P<0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29253907), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglin/CD105
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human endoglin cDNA encodes 658 amino acids including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) endoglin (6, 7). The human endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine, and canine endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with Activin A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to anti-angiogenic activity (15).
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- ten Dijke, P. et al. (2008) Angiogenesis 11:79.
- Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
- Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
- Moody, J.L. et al. (2007) Stem Cells 25:2809.
- Velasco, S. et al. (2008) J. Cell Sci. 121:913.
- Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
- Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
- Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
- Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
- Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
- Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
- Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
- Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.
- Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Product Datasheets
Citations for Human Endoglin/CD105 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Endoglin and squamous cell carcinomas
Authors: Sarah K. Hakuno, Stefanus G. T. Janson, Marjolijn D. Trietsch, Manon de Graaf, Eveline de Jonge-Muller, Stijn Crobach et al.
Frontiers in Medicine
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Prostate-Specific Membrane Antigen Targeted Pet/CT Imaging in Patients with Colon, Gastric and Pancreatic Cancer
Authors: FA Vuijk, F Kleiburg, WA Noortman, L Heijmen, S Feshtali S, FHP van Velden, VM Baart, SS Bhairosing, BD Windhorst, LJAC Hawinkels, P Dibbets-Sc, N Bouwman, SALP Crobach, A Fariña-Sar, AWKS Marinelli, DE Oprea-Lage, RJ Swijnenbur, F Smit, AL Vahrmeijer, LF de Geus-Oe, DE Hilling, M Slingerlan
Cancers, 2022-12-15;14(24):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Targeting Endoglin Expressing Cells in the Tumor Microenvironment Does Not Inhibit Tumor Growth in a Pancreatic Cancer Mouse Model
Authors: MJA Schoonderw, SK Hakuno, M Sassen, EB Kuhlemaije, M Paauwe, M Slingerlan, MF Fransen, LJAC Hawinkels
OncoTargets and Therapy, 2021-10-29;14(0):5205-5220.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Inhibiting DPP4 in a mouse model of HHT1 results in a shift towards regenerative macrophages and reduces fibrosis after myocardial infarction
Authors: CKE Dingenouts, W Bakker, K Lodder, KC Wiesmeijer, AT Moerkamp, JA Maring, HM Arthur, AM Smits, MJ Goumans
PLoS ONE, 2017-12-18;12(12):e0189805.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Endoglin targeting inhibits tumor angiogenesis and metastatic spread in breast cancer
Authors: M Paauwe, R C Heijkants, C H Oudt, G W van Pelt, C Cui, C P Theuer et al.
Oncogene
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Lymphangiogenesis and angiogenesis during human fetal pancreas development
Authors: Matthias S Roost, Liesbeth van Iperen, Ana de Melo Bernardo, Christine L Mummery, Françoise Carlotti, Eelco JP de Koning et al.
Vascular Cell
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