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Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

Catalog #: MAB9577
2 Citations Datasheet
Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB9577-100
MAB9577-SP
Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EGFR (Research Grade Cetuximab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGFR based on Cetuximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Cetuximab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu1
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human EGF R/ErbB1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of EGF R/ErbB1 antibody in A431 human epithelial carcinoma cell line antibody by Flow Cytometry. View Larger

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Monoclonal Antibody (Catalog # MAB9577, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

Cetuximab is a biosimilar directed against the extracellular domain of EGFR. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB9626. The biotinylated antibody (catalog number FAB9577B) can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.

Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Citations for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Detection of EGFR and its Activity State in Plasma CD63-EVs from Glioblastoma Patients: Rapid Profiling using an Anion Exchange Membrane Sensor
    Authors: Maniya, NH;Kumar, S;Franklin, JL;Higginbotham, JN;Scott, AM;Gan, HK;Coffey, RJ;Senapati, S;Chang, HC;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Extracellular Vesicles
    Applications: Bioassay
  2. Engineered CD8+ T cell-derived extracellular vesicles induce enhanced anti-cancer efficacy and targeting to lung cancer cells
    Authors: Cho, H;Jung, I;Ju, H;Baek, MC;Yea, K;
    Cytokine
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

  1. What is a Biosimilar antibody?

    • A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  2. Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    • This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

  3. Is the light chain of Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog # MAB9577,  kappa or lamba?

    • MAB9577 is a kappa light chain IgG1.

  4. What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?

    • Catalog #s MAB9577 and FAB9577 have a kappa light chain.

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