Home / EGFR / Human EGFR Antibody

Human EGFR Antibody

Catalog #: AF231
30 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
AF231
AF231-SP
Detection of Human EGFR by Western Blot.
15 Images
Product Details
Citations (30)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EGFR Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGFR in ELISAs and Western blots. In sandwich ELISAs, approximately 3% cross-reactivity with recombinant mouse EGFR is observed and less than 0.1% cross-reactivity with recombinant human (rh) ErbB2 and rhErbB3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EGFR
Leu25-Ser645
Accession # CAA25240
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
12.5 µg/mL
A431 human epithelial carcinoma cell line
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
1-15 µg/mL
See below
Immunoprecipitation
1 µg/mL
A431 human epithelial carcinoma cell line, see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human skin
Immunocytochemistry
1-15 µg/mL
See below

Human EGFR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human EGFR Biotinylated Antibody (Catalog # BAF231)

Standard: Recombinant Human EGFR Protein (Catalog # 1095-ER)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EGFR antibody by Western Blot. View Larger

Detection of Human EGFR by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of EGFR antibody in A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of EGFR in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry EGFR antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

EGFR in A431 Human Cell Line. EGFR was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry EGFR antibody in Human Skin by Immunohistochemistry (IHC-Fr). View Larger

EGFR in Human Skin. EGFR was detected in immersion fixed frozen sections of human skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Detection of Mouse EGFR by Western Blot View Larger

Detection of Mouse EGFR by Western Blot Crizotinib combined with mutant-selective EGFR-TKI overcomes multiple resistances to EGFR-TKI invivo.(A) SCID mice-bearing H1975/Vec- or H1975/HGF- tumors were administered WZ4002 (25 mg/kg) and/or crizotinib (10, 25mg/kg) once daily for 6 to 20 days. Tumor volume was measured using calipers on the indicated days. Mean ± SE tumor volumes are shown for groups of 5 mice. *, P < 0.05 versus control; ✝, P < 0.05 versus WZ4002 by one-way ANOVA. (B) H1975/Vec- or H1975/HGF- tumors were resected from the mice 3 hours after administration of WZ4002 (25mg/kg) and/or crizotinib (10, 25 mg/kg), and the relative levels of proteins in the tumor lysates were determined by western blot analysis. (C) Representative images of H1975/Vec- and H1975/HGF- tumors immunohistochemically stained with antibodies to human Ki-67, and stained with both DAPI (nuclear stain) and TUNEL (FITC). Bar, 200 μm. (D) Quantification of proliferative cells, as determined by the Ki-67-positive proliferation index (percentage of Ki-67-positive cells). Quantification of apoptotic cells, as determined by the TUNEL assay as described in Materials and Methods. Columns, mean of five areas; bars, SD *, P < 0.05 versus of H1975/Vec-tumors; ✝, P < 0.05 versus H1975/HGF-tumors by one-way ANOVA. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0084700), licensed under a CC-BY license. Not internally tested by R&D Systems.

Simple Western View Larger

Detection of Human EGFR by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 4.2 mg/mL. A specific band was detected for EGFR at approximately 229 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Human EGFR by Western Blot View Larger

Detection of Human EGFR by Western Blot Crizotinib reduces Met phosphorylation and combined treatment with a new generation EGFR-TKI inhibits downstream pathways even in the presence of HGF.H1975 and H1975/HGF cells were incubated with crizotinib (300 nmol/L) and/or afatinib (300 nmol/L) (A, B) or WZ4002 (300 nmol/L) (C, D), for 1 hour. After stimulation with HGF (10 ng/mL) for 10 minutes, the cell lysates were harvested and the phosphorylation of indicated proteins was determined by western blot analysis. Each sample was assayed in triplicate, with each experiment repeated at least 3 times independently. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0084700), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human EGFR by Western Blot View Larger

Detection of Human EGFR by Western Blot Crizotinib reduces Met phosphorylation and combined treatment with a new generation EGFR-TKI inhibits downstream pathways even in the presence of HGF.H1975 and H1975/HGF cells were incubated with crizotinib (300 nmol/L) and/or afatinib (300 nmol/L) (A, B) or WZ4002 (300 nmol/L) (C, D), for 1 hour. After stimulation with HGF (10 ng/mL) for 10 minutes, the cell lysates were harvested and the phosphorylation of indicated proteins was determined by western blot analysis. Each sample was assayed in triplicate, with each experiment repeated at least 3 times independently. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0084700), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human EGFR by Western Blot View Larger

Detection of Human EGFR by Western Blot Crizotinib reduces Met phosphorylation and combined treatment with a new generation EGFR-TKI inhibits downstream pathways even in the presence of HGF.H1975 and H1975/HGF cells were incubated with crizotinib (300 nmol/L) and/or afatinib (300 nmol/L) (A, B) or WZ4002 (300 nmol/L) (C, D), for 1 hour. After stimulation with HGF (10 ng/mL) for 10 minutes, the cell lysates were harvested and the phosphorylation of indicated proteins was determined by western blot analysis. Each sample was assayed in triplicate, with each experiment repeated at least 3 times independently. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0084700), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human EGFR by Western Blot View Larger

Detection of Human EGFR by Western Blot Crizotinib reduces Met phosphorylation and combined treatment with a new generation EGFR-TKI inhibits downstream pathways even in the presence of HGF.H1975 and H1975/HGF cells were incubated with crizotinib (300 nmol/L) and/or afatinib (300 nmol/L) (A, B) or WZ4002 (300 nmol/L) (C, D), for 1 hour. After stimulation with HGF (10 ng/mL) for 10 minutes, the cell lysates were harvested and the phosphorylation of indicated proteins was determined by western blot analysis. Each sample was assayed in triplicate, with each experiment repeated at least 3 times independently. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0084700), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse EGFR by Western Blot View Larger

Detection of Mouse EGFR by Western Blot Crizotinib combined with irreversible EGFR-TKI overcomes multiple resistances to EGFR-TKI in vivo.(A) SCID mice-bearing H1975/Vec- or H1975/HGF- tumors were administered afatinib (25 mg/kg) and/or crizotinib (10mg/kg) once daily for 6 to 20 days. Tumor volume was measured using calipers on the indicated days. Mean ± SE tumor volumes are shown for groups of 5 mice. *, P < 0.05 versus control; ✝, P < 0.05 versus afatinib (25 mg/kg) by one-way ANOVA. (B) H1975/Vec- or H1975/HGF- tumors were resected from the mice 3 hours after administration of afatinib (25mg/kg) and/or crizotinib (10 mg/kg), and the relative levels of proteins in the tumor lysates were determined by western blot analysis. (C) Representative images of H1975/Vec- and H1975/HGF tumors immunohistochemically stained with antibodies to human Ki-67, and stained with both DAPI (nuclear stain) and TUNEL (FITC). Bar, 200 μm. (D) Quantification of proliferative cells, as determined by their Ki-67-positive proliferation index (percentage of Ki-67-positive cells). Quantification of apoptotic cells, as determined by the TUNEL assay as described in Materials and Methods. Columns, mean of five areas; bars, SD. *, P < 0.05 versus H1975/Vec-tumors; ✝, P < 0.05 versus control of H1975/HGF-tumors by one-way ANOVA. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0084700), licensed under a CC-BY license. Not internally tested by R&D Systems.

In-situ Hybridization View Larger

Detection of EGFR in Human Skin. Formalin-fixed paraffin-embedded tissue sections of human skin were probed for EGFR mRNA (ACD RNAScope Probe, catalog # 310061; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using goat anti-human EGFR polyclonal antibody (R&D Systems catalog # AF231) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes.

Western Blot Detection of Human Human EGFR Antibody by Western Blot View Larger

Detection of Human Human EGFR Antibody by Western Blot Crizotinib reduces Met phosphorylation and combined treatment with a new generation EGFR-TKI inhibits downstream pathways even in the presence of HGF.H1975 and H1975/HGF cells were incubated with crizotinib (300 nmol/L) and/or afatinib (300 nmol/L) (A, B) or WZ4002 (300 nmol/L) (C, D), for 1 hour. After stimulation with HGF (10 ng/mL) for 10 minutes, the cell lysates were harvested and the phosphorylation of indicated proteins was determined by western blot analysis. Each sample was assayed in triplicate, with each experiment repeated at least 3 times independently. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24386407), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Human EGFR Antibody by Western Blot View Larger

Detection of Mouse Human EGFR Antibody by Western Blot Crizotinib combined with mutant-selective EGFR-TKI overcomes multiple resistances to EGFR-TKI invivo.(A) SCID mice-bearing H1975/Vec- or H1975/HGF- tumors were administered WZ4002 (25 mg/kg) and/or crizotinib (10, 25mg/kg) once daily for 6 to 20 days. Tumor volume was measured using calipers on the indicated days. Mean ± SE tumor volumes are shown for groups of 5 mice. *, P < 0.05 versus control; ✝, P < 0.05 versus WZ4002 by one-way ANOVA. (B) H1975/Vec- or H1975/HGF- tumors were resected from the mice 3 hours after administration of WZ4002 (25mg/kg) and/or crizotinib (10, 25 mg/kg), and the relative levels of proteins in the tumor lysates were determined by western blot analysis. (C) Representative images of H1975/Vec- and H1975/HGF- tumors immunohistochemically stained with antibodies to human Ki-67, and stained with both DAPI (nuclear stain) and TUNEL (FITC). Bar, 200 μm. (D) Quantification of proliferative cells, as determined by the Ki-67-positive proliferation index (percentage of Ki-67-positive cells). Quantification of apoptotic cells, as determined by the TUNEL assay as described in Materials and Methods. Columns, mean of five areas; bars, SD *, P < 0.05 versus of H1975/Vec-tumors; ✝, P < 0.05 versus H1975/HGF-tumors by one-way ANOVA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24386407), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human Human EGFR Antibody by Western Blot View Larger

Detection of Human Human EGFR Antibody by Western Blot Crizotinib reduces Met phosphorylation and combined treatment with a new generation EGFR-TKI inhibits downstream pathways even in the presence of HGF.H1975 and H1975/HGF cells were incubated with crizotinib (300 nmol/L) and/or afatinib (300 nmol/L) (A, B) or WZ4002 (300 nmol/L) (C, D), for 1 hour. After stimulation with HGF (10 ng/mL) for 10 minutes, the cell lysates were harvested and the phosphorylation of indicated proteins was determined by western blot analysis. Each sample was assayed in triplicate, with each experiment repeated at least 3 times independently. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24386407), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha  in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis (1 - 3).

References
  1. Daly, R.J. (1999) Growth Factors, 16:255.
  2. Schlessinger, J. (2000) Cell. 103:211.
  3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Product Datasheets

You must select a language.

x
Product Datasheet

Citations for Human EGFR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Soluble fms-Like Tyrosine Kinase 1 Localization in Renal Biopsies of CKD
    Authors: ZK Zsengellér, A Lo, M Tavasoli, E Pernicone, SA Karumanchi, S Rosen
    Kidney Int Rep, 2019-08-14;4(12):1735-1741.
  2. The EGFR phosphatase RPTP gamma is a redox‐regulated suppressor of promigratory signaling
    Authors: Maitreyi S Joshi, Angel Stanoev, Jan Huebinger, Birga Soetje, Veronika Zorina, Lisaweta Ro beta mannek et al.
    The EMBO Journal
  3. An atlas of late prenatal human neurodevelopment resolved by single-nucleus transcriptomics
    Authors: SI Ramos, ZM Mussa, EN Falk, B Pai, B Giotti, K Allette, P Cai, F Dekio, R Sebra, KG Beaumont, AM Tsankov, NM Tsankova
    Nature Communications, 2022-12-12;13(1):7671.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC/IF
  4. Integrated Analytical System for Clinical Single‐Cell Analysis
    Authors: Hannah M. Peterson, Lip Ket Chin, Yoshi Iwamoto, Juhyun Oh, Jonathan C. T. Carlson, Hakho Lee et al.
    Advanced Science
  5. Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression
    Authors: DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger
    The Journal of Biological Chemistry, 2022-05-13;0(0):102029.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  6. Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias
    Authors: C Hu, CA Leche, A Kiyatkin, Z Yu, SE Stayrook, KM Ferguson, MA Lemmon
    Nature, 2022-02-09;602(7897):518-522.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Developmental Origins of Human Cortical Oligodendrocytes and Astrocytes
    Authors: Lin Yang, Zhenmeiyu Li, Guoping Liu, Xiaosu Li, Zhengang Yang
    Neuroscience Bulletin
  8. EGFR transactivates RON to drive oncogenic crosstalk
    Authors: Carolina Franco Nitta, Ellen W Green, Elton D Jhamba, Justine M Keth, Iraís Ortiz-Caraveo, Rachel M Grattan et al.
    eLife
  9. Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window
    Authors: T Egnuni, N Ingram, I Mirza, PL Coletta, JR McLaughlan
    Pharmaceutics, 2021-10-09;13(10):.
    Species: Human
    Sample Types: Whole Cell
    Applications: IF
  10. Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation
    Authors: R Chakrabort, K Vickery, C Darido, S Ranganatha, H Hu
    International Journal of Molecular Sciences, 2021-08-12;22(16):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
    Authors: S Sugita, M Yamato, T Hatabu, Y Kataoka
    Scientific Reports, 2021-05-17;11(1):9668.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. EGFR inhibition blocks cancer stem cell clustering and lung metastasis of triple negative breast cancer
    Authors: X Liu, V Adorno-Cru, YF Chang, Y Jia, M Kawaguchi, NK Dashzeveg, R Taftaf, EK Ramos, EJ Schuster, L El-Shennaw, D Patel, Y Zhang, M Cristofani, H Liu
    Theranostics, 2021-04-30;11(13):6632-6643.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation
  13. Development of an immuno-wall device for the rapid and sensitive detection of EGFR mutations in tumor tissues resected from lung cancer patients
    Authors: N Yogo, T Hase, T Kasama, K Nishiyama, N Ozawa, T Hatta, H Shibata, M Sato, K Komeda, N Kawabe, K Matsuoka, TF Chen-Yoshi, N Kaji, M Tokeshi, Y Baba, Y Hasegawa
    PLoS ONE, 2020-11-16;15(11):e0241422.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Detection
  14. Systems Modeling Identifies Divergent Receptor Tyrosine Kinase Reprogramming to MAPK Pathway Inhibition
    Authors: Allison M. Claas, Lyla Atta, Simon Gordonov, Aaron S. Meyer, Douglas A. Lauffenburger
    Cellular and Molecular Bioengineering
  15. Interdependence between EGFR and Phosphatases Spatially Established by Vesicular Dynamics Generates a Growth Factor Sensing and Responding Network
    Authors: Angel Stanoev, Amit Mhamane, Klaus C. Schuermann, Hernán E. Grecco, Wayne Stallaert, Martin Baumdick et al.
    Cell Systems
  16. A conformational sensor based on genetic code expansion reveals an autocatalytic component in EGFR activation
    Authors: Martin Baumdick, Márton Gelléri, Chayasith Uttamapinant, Václav Beránek, Jason W. Chin, Philippe I. H. Bastiaens
    Nature Communications
  17. Identification of a novel anoikis signalling pathway using the fungal virulence factor gliotoxin
    Authors: F Haun, S Neumann, L Peintner, K Wieland, J Habicht, C Schwan, K Østevold, MM Koczorowsk, M Biniossek, M Kist, H Busch, M Boerries, RJ Davis, U Maurer, O Schilling, K Aktories, C Borner
    Nat Commun, 2018-08-30;9(1):3524.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes
    Authors: A Klaesson, K Grannas, T Ebai, J Heldin, B Koos, M Leino, D Raykova, J Oelrich, L Arngården, O Söderberg, U Landegren
    Sci Rep, 2018-03-29;8(1):5400.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Cerebellar Granule Cell Replenishment Post-Injury by Adaptive Reprogramming of Nestin+ Progenitors
    Authors: Alexandre Wojcinski, Andrew K. Lawton, N Sumru. Bayin, Zhimin Lao, Daniel N. Stephen, Alexandra L. Joyner
    Nature Neuroscience
  20. Soluble fms-like tyrosine kinase 1 promotes angiotensin II sensitivity in preeclampsia
    Authors: Suzanne D Burke
    J Clin Invest, 2016-06-06;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. EGF-dependent re-routing of vesicular recycling switches spontaneous phosphorylation suppression to EGFR signaling
    Authors: Martin Baumdick, Yannick Brüggemann, Malte Schmick, Georgia Xouri, Ola Sabet, Lloyd Davis et al.
    eLife
  22. Aptamer targeting EGFRvIII mutant hampers its constitutive autophosphorylation and affects migration, invasion and proliferation of glioblastoma cells
    Authors: Simona Camorani, Elvira Crescenzi, David Colecchia, Andrea Carpentieri, Angela Amoresano, Monica Fedele et al.
    Oncotarget
  23. Multichannel Imaging to Quantify Four Classes of Pharmacokinetic Distribution in Tumors
    Authors: Sumit Bhatnagar, Emily Deschenes, Jianshan Liao, Cornelius Cilliers, Greg M. Thurber
    Journal of Pharmaceutical Sciences
  24. Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips†
    Authors: Christopher M. Earhart, Casey E. Hughes, Richard S. Gaster, Chin Chun Ooi, Robert J. Wilson, Lisa Y. Zhou et al.
    Lab Chip
  25. Ability of the Met kinase inhibitor crizotinib and new generation EGFR inhibitors to overcome resistance to EGFR inhibitors.
    Authors: Nanjo, Shigeki, Yamada, Tadaaki, Nishihara, Hiroshi, Takeuchi, Shinji, Sano, Takako, Nakagawa, Takayuki, Ishikawa, Daisuke, Zhao, Lu, Ebi, Hiromich, Yasumoto, Kazuo, Matsumoto, Kunio, Yano, Seiji
    PLoS ONE, 2013-12-26;8(12):e84700.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts.
    Authors: Sarup J, Jin P, Turin L, Bai X, Beryt M, Brdlik C, Higaki JN, Jorgensen B, Lau FW, Lindley P, Liu J, Ni I, Rozzelle J, Kumari R, Watson SA, Zhang J, Shepard HM
    Mol. Cancer Ther., 2008-10-01;7(10):3223-36.
    Species: Human
    Sample Types: Cell Lysates, Recombinant Protein
    Applications: ELISA Development, Western Blot
  27. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  28. FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival.
    Authors: Kunii K, Davis L, Gorenstein J, Hatch H, Yashiro M, Di Bacco A, Elbi C, Lutterbach B
    Cancer Res., 2008-04-01;68(7):2340-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  29. Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
    Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
    Eur J Vasc Endovasc Surg, 2006-01-20;31(5):516-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  30. Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles
    Authors: E Reátegui, KE van der Vo, CP Lai, M Zeinali, NA Atai, B Aldikacti, FP Floyd, A H Khankhel, V Thapar, FH Hochberg, LV Sequist, BV Nahed, B S Carter, M Toner, L Balaj, D T Ting, XO Breakefiel, SL Stott
    Nat Commun, 2018-01-12;9(1):175.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human EGFR Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human EGFR Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human EGFR Antibody
By Anonymous on 07/31/2018
Application: WB Sample Tested: HT-29 human colon adenocarcinoma cell line Species: Human
Western Blot Human EGFR Antibody AF231