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Human DPPIV/CD26 Antibody

Catalog #: MAB1180
6 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB1180-SP
MAB1180-500
MAB1180-100
Detection of Human DPPIV/CD26 by Western Blot.
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Product Details
Citations (6)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DPPIV/CD26 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DPPIV/CD26 in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin A or recombinant mouse DPPIV is observed.
Source
Monoclonal Rat IgG2A Clone # 222113
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26
Asp34-Pro766
Accession # Q53TN1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human DPPIV/CD26 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human DPPIV/CD26 Biotinylated Antibody (Catalog # BAF1180)

Standard: Recombinant Human DPPIV/CD26 Protein, CF (Catalog # 1180-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DPPIV/CD26 antibody by Western Blot. View Larger

Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # MAB1180) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of DPPIV/CD26 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of DPPIV/CD26 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Rat Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # MAB1180, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Anti-mouse PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression. DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

References
  1. Misumi and Ikehara (1998) in Handbook of Proteolytic Enzymes. Barrett, et al. (eds) San Diego: Academic Press, p. 378.
  2. Ikehara, et al. (1994) Methods Enzymol. 244:215.
  3. Marguet, et al. (1992) J. Biol. Chem. 267:2200.
  4. Bernard, et al. (1994) Biochemistry 33:15204.
  5. Viver, et al. (1991) J. Immunol. 147:447.
  6. Callebaut, et al. (1993) Science 262:2045.
  7. Kameoka, et al. (1993) Science 261:466.
  8. Ohtsuki, et al. (1998) FEBS Lett. 431:236.
  9. Proost, et al. (1999) J. Biol. Chem. 274:3988.
  10. Hinke, et al. (2000) J. Biol. Chem. 275:3827.
  11. Wrenger, et al. (2000) FEBS Lett. 466:155.
Long Name
Dipeptidyl-peptidase IV
Entrez Gene IDs
1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)
Alternate Names
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

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Citations for Human DPPIV/CD26 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Differentiation of immortalized human multi-lineage progenitor to alveolar type 2-like cells: angiotensin-converting enzyme 2 expression and binding of severe acute respiratory syndrome coronavirus 2 spike and spike 1 proteins
    Authors: Collins DP, Osborn MJ, Steer CJ. et al.
    Cytotherapy
  2. Senescence-like phenotype in post-mitotic cells of mice entering middle age
    Authors: Marco Raffaele, Kristina Kovacovicova, Francesca Bonomini, Rita Rezzani, Jan Frohlich, Manlio Vinciguerra
    Aging (Albany NY)
  3. Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
    Authors: Chen Lu, Joshua L. Wonsidler, Jianwei Li, Yanming Du, Timothy Block, Brian Haab et al.
    Journal of Visualized Experiments
  4. Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4
    Authors: AK Haverkamp, A Lehmbecker, I Spitzbarth, W Widagdo, BL Haagmans, J Segalés, J Vergara-Al, A Bensaid, JMA van den Br, ADME Osterhaus, W Baumgärtne
    Sci Rep, 2018-06-27;8(1):9778.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Engineering a stable CHO cell line for the expression of a MERS-coronavirus vaccine antigen
    Authors: MP Nyon, L Du, CK Tseng, CA Seid, J Pollet, KS Naceanceno, A Agrawal, A Algaissi, BH Peng, W Tai, S Jiang, ME Bottazzi, U Strych, PJ Hotez
    Vaccine, 2018-02-26;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Involvement of DPP-IV catalytic residues in enzyme-saxagliptin complex formation.
    Authors: Metzler WJ, Yanchunas J, Weigelt C
    Protein Sci., 2008-02-01;17(2):240-50.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development

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