Human DOK3 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB4795S Datasheet

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FAB4795S-100UG

All DOK3 Antibodies

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Human DOK3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DOK3 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG2b Clone # 642316

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human DOK3
Gly181-Lys271
Accession # Q7L591

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DOK3

DOK3 (downstream of kinase 3) is a 53 kDa (predicted) member of the DOK family of cytoplasmic adaptor proteins and is expressed in B cells and macrophages. Human DOK3 cDNA encodes 496 amino acids (aa) including one pleckstrin homology region (aa 63‑179) and an IRS-type phosphotyrosine binding domain (PTB) (aa 213‑317). Alternate splicing generates 330, 228 and 216 aa isoforms with N-terminal and C-terminal truncations and substitutions. Human DOK3 shares approximately 80% aa sequence identity with mouse and rat DOK3 within aa 181‑271, which are common to all four isoforms. Phosphorylation of tyrosine residues in DOK3 regulates its interaction with SH2 and SH3 containing proteins, leading to inhibition of signaling from immunoreceptors.

Long Name

Downstream of Tyrosine Kinase 3

Entrez Gene IDs

79930 (Human); 27261 (Mouse); 306760 (Rat)

Alternate Names

docking protein 3; DOK3; DOKL; Dok-like protein; Downstream of tyrosine kinase 3; FLJ22570; FLJ39939