Human DNAM-1/CD226
Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8⁺ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung (11). CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation (12). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 13-15).

Product Datasheets

Related Research Areas

• B Cell Activation
• Co-stimulatory and Co-inhibitory Molecules
• ITIM/ITAM Immunoreceptors and Related Molecules
• Monocyte Markers
• Nectin and Nectin-like Binding Receptors and Ligands
• Platelet Activation Markers
• Search Immune Checkpoint Targets
• T Cell Migration/Adhesion