Human DDR2 Antibody Summary
Gln24-Arg399
Accession # Q16832
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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DDR2 in Human Lung. DDR2 was detected in immersion fixed paraffin-embedded sections of human lung using 25 µg/mL Mouse Anti-Human DDR2 Monoclonal Antibody (Catalog # MAB2538) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to to cells in connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DDR2
DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3‑5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10‑12).
- Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
- Karn, T. et al. (1993) Oncogene 8:3433.
- Vogel, W. et al. (1997) Mol. Cell 1:13.
- Leitinger, B. (2003) J. Biol. Chem. 278:16761.
- Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
- Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
- Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
- Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
- Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
- Xu, L. et al. (2005) J. Biol. Chem. 280:548.
- Wang, J. et al. (2002) J. Autoimmun. 19:161.
- Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.
Product Datasheets
Citations for Human DDR2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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The collagen structure of C1q induces wound healing by engaging discoidin domain receptor 2
Authors: Ria Aryani Hayuningtyas, Myeonggil Han, Seoyeon Choi, Man Sup Kwak, In Ho Park, Ji-Hyun Lee et al.
Molecular Medicine
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TWIST1 induces expression of discoidin domain receptor 2 to promote ovarian cancer metastasis
Authors: WR Grither, LM Divine, EH Meller, DJ Wilke, RA Desai, AJ Loza, P Zhao, A Lohrey, GD Longmore, KC Fuh
Oncogene, 2018-01-19;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes
Authors: H Mogami, AH Kishore, RA Word
Sci Rep, 2018-01-12;8(1):696.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Tyrosine kinase discoidin domain receptors DDR1 and DDR2 are coordinately deregulated in triple-negative breast cancer
Authors: Kathy A. Toy, Rajeshwari R. Valiathan, Fernando Núñez, Kelley M. Kidwell, Maria E. Gonzalez, Rafael Fridman et al.
Breast Cancer Research and Treatment
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Regulation of collagen fibrillogenesis by cell-surface expression of kinase dead DDR2.
Authors: Blissett AR, Garbellini D, Calomeni EP, Mihai C, Elton TS, Agarwal G
J. Mol. Biol., 2008-10-30;385(3):902-11.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Increased expression of discoidin domain receptor 2 is linked to the degree of cartilage damage in human knee joints: a potential role in osteoarthritis pathogenesis.
Authors: Sunk IG, Bobacz K, Hofstaetter JG, Amoyo L, Soleiman A, Smolen J, Xu L, Li Y
Arthritis Rheum., 2007-11-01;56(11):3685-92.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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