Human DDR1 Antibody Summary
Asp21-Thr416
Accession # Q08345
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human DDR1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DDR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2396) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for DDR1 at approximately 100 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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DDR1 in Human Breast Cancer Tissue. DDR1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human DDR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2396) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human DDR1 by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for DDR1 at approximately 137 kDa (as indicated) using 10 µg/mL of Goat Anti-Human DDR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2396) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DDR1
DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120-140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases. Mature human DDR2 consists of a 398 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 27 aa transmembrane segment, and a 470 aa cytoplasmic region with a tyrosine kinase domain. Within the ECD, human DDR1 shares 53% aa sequence identity with human DDR2 and 93% with mouse and rat DDR1. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers. Compared to isoform DDR1b, DDR1a lacks 37 aa’s that include a Shc-interacting NPxY motif in the cytoplasmic juxtamembrane region. Two additional kinase deficient splice forms are expressed in colon cancer. The discoidin-like domain mediates binding to collagens I-V. DDR1 selectively recognizes the triple helical structure of collagen. It is expressed on the cell surface as a dimer which can include different isoforms. DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis. The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization. Collagen binding induces prolonged autophosphorylation, including the NPxY motif. Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment. TIMP3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF. Over-expression of DDR1a promotes MMP-2 activation and results in an increased invasiveness of a glioblastoma cell line; DDR1b does not.
Product Datasheets
Citations for Human DDR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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DDR1 promotes breast tumor growth by suppressing antitumor immunity
Authors: X Zhong, W Zhang, T Sun
Oncol. Rep., 2019-09-27;0(0):.
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Glomerular basement membrane deposition of collagen ?1(III) in Alport glomeruli by mesangial filopodia injures podocytes via aberrant signaling through DDR1 and integrin ?2?1
Authors: Madison J, Wilhelm K, Meehan DT et al.
The Journal of Pathology
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Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
Nature, 2021-11-03;0(0):.
Species: Human
Sample Types: Cell Culture Supernates, Whole Tissue
Applications: ELISA Capture, IHC -
Serum N-terminal DDR1: A Novel Diagnostic Marker of Liver Fibrosis Severity
Authors: Yuxin Zhang, Yujie Zhang, Huifang Liang, Zeng Zhuo, Pan Fan, Yifa Chen et al.
Journal of Clinical and Translational Hepatology
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DDR1-induced neutrophil extracellular traps drive pancreatic cancer metastasis
Authors: Jenying Deng, Yaan Kang, Chien-Chia Cheng, Xinqun Li, Bingbing Dai, Matthew H. Katz et al.
JCI Insight
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Collagen induces activation of DDR1 through lateral dimer association and phosphorylation between dimers
Authors: Victoria Juskaite, David S Corcoran, Birgit Leitinger
eLife
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Cancer Cell Invasion in Three-dimensional Collagen Is Regulated Differentially by Galpha13 Protein and Discoidin Domain Receptor 1-Par3 Protein Signaling.
Authors: Chow C, Ebine K, Knab L, Bentrem D, Kumar K, Munshi H
J Biol Chem, 2015-11-20;291(4):1605-18.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
SAR Studies of Exosite-Binding Substrate-Selective Inhibitors of A Disintegrin And Metalloprotease 17 (ADAM17) and Application as Selective in Vitro Probes
Authors: Anna M. Knapinska, Daniela Dreymuller, Andreas Ludwig, Lyndsay Smith, Vladislav Golubkov, Anjum Sohail et al.
Journal of Medicinal Chemistry
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ADAM10 controls collagen signaling and cell migration on collagen by shedding the ectodomain of discoidin domain receptor 1 (DDR1)
Authors: Yasuyuki Shitomi, Ida B. Thøgersen, Noriko Ito, Birgit Leitinger, Jan J. Enghild, Yoshifumi Itoh
Molecular Biology of the Cell
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Shedding of discoidin domain receptor 1 by membrane-type matrix metalloproteinases.
Authors: Fu, Hsueh-Li, Sohail, Anjum, Valiathan, Rajeshwa, Wasinski, Benjamin, Kumarasiri, Malika, Mahasenan, Kiran V, Bernardo, M Margar, Tokmina-Roszyk, Dorota, Fields, Gregg B, Mobashery, Shahriar, Fridman, Rafael
J Biol Chem, 2013-03-21;288(17):12114-29.
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Cell Lysates
Applications: Western Blot -
Type II collagen levels correlate with mineralization by articular cartilage vesicles.
Authors: Jubeck B, Muth E, Gohr CM, Rosenthal AK
Arthritis Rheum., 2009-09-01;60(9):2741-6.
Species: Porcine
Sample Types: Whole Tissue
Applications: Western Blot -
Glycosylation at Asn211 regulates the activation state of the discoidin domain receptor 1 (DDR1).
Authors: Fu H, Valiathan R, Payne L, Kumarasiri M, Mahasenan K, Mobashery S, Huang P, Fridman R
J Biol Chem, 2014-02-07;289(13):9275-87.
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