Human CXCL4/PF4 Antibody Summary
Glu32-Ser101
Accession # P02776.2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CXCL4/PF4 by Western Blot. Western blot shows lysates of human spleen tissue and human platelets. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7952) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of CXCL4/PF4 in PBMC monocytes by Flow Cytometry. PBMC monocytes were stained with Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7952, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of CXCL4/PF4 in Platelets by Flow Cytometry. Platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 APC‑conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7952) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL4/PF4
CXCL4, also known as Platelet Factor 4 (PF4), is an 8 kDa CXC chemokine that is stored in platelet alpha -granules as a
homotetramer and secreted abundantly during platelet activation. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein that includes granule targeting and
heparin-binding sequences. CXCL4 has homology with IL-8 and beta -thromboglobulin and can form heteromultimers with IL-8. Mature human and mouse CXCL4 share 76% aa identity. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in
alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, has anti-angiogenic activity and promotes granule Protein C activation. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.
- Poncz, M. et al. (1987) Blood 69:219.
- Scheuerer, B. et al. (2000) Blood 95:1158.
- Perollet, C. et al. (1998) Blood 91:3289.
- Petersen, F. et al. (1998) J. Immunol. 161:4347.
- Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
- Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
Product Datasheets
Citations for Human CXCL4/PF4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Platelet factors attenuate inflammation and rescue cognition in ageing
Authors: Schroer, AB;Ventura, PB;Sucharov, J;Misra, R;Chui, MKK;Bieri, G;Horowitz, AM;Smith, LK;Encabo, K;Tenggara, I;Couthouis, J;Gross, JD;Chan, JM;Luke, A;Villeda, SA;
Nature
Species: Human
Sample Types: Platelet-Rich Plasma
Applications: Western Blot -
Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI.
Authors: Ollivier V, Syvannarath V, Gros A, Butt A, Loyau S, Jandrot-Perrus M, Ho-Tin-Noe B
PLoS ONE, 2014-08-12;9(8):e104712.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CXCL4L1–fibstatin cooperation inhibits tumor angiogenesis, lymphangiogenesis and metastasis
Authors: A.C. Prats, L. Van den Berghe, A. Rayssac, N. Ainaoui, F. Morfoisse, F. Pujol et al.
Microvascular Research
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Functional divergence between 2 chemokines is conferred by single amino acid change.
Authors: Dubrac A, Quemener C, Lacazette E, Lopez F, Zanibellato C, Wu WG, Bikfalvi A, Prats H
Blood, 2010-08-05;116(22):4703-11.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay -
A new obligate CXCL4-CXCL12 heterodimer for studying chemokine heterodimer activities and mechanisms
Authors: KTP Nguyen, B Volkman, D Dréau, IV Nesmelova
Scientific Reports, 2022-10-13;12(1):17204.
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