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Human CXCL12/SDF-1 Antibody

Catalog #: AF-310-NA
24 Citations Datasheet
Catalog # Availability Size / Price Qty
AF-310-NA
AF-310-SP
Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCL12/SDF‑1 Antibody.
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Citations (24)
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Human CXCL12/SDF-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL12/SDF-1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human/Feline CXCL12/SDF-1 beta aa 19-93 (Catalog # 2716-SD)
Recombinant Human/Feline CXCL12/SDF-1 beta aa 22-93 (Catalog # 351-FS)
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS)
Neutralization
Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 2 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCL12/SDF‑1 Antibody. View Larger

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCL12/SDF‑1 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL12/SDF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND50 is typically 1.5-6.0 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL12/SDF-1

CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.

Entrez Gene IDs
6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)
Alternate Names
chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1

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Citations for Human CXCL12/SDF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Estrogen receptor alpha promotes lung cancer cell invasion via increase of and cross‐talk with infiltrated macrophages through the CCL2/CCR2/MMP9 and CXCL12/CXCR4 signaling pathways
    Authors: Miao He, Weiwei Yu, Chawnshang Chang, Hiroshi Miyamoto, Xiaohong Liu, Ke Jiang et al.
    Molecular Oncology
  2. Aberrant Proliferation in CXCR7+ Endothelial Cells via Degradation of the Retinoblastoma Protein
    Authors: Jennifer E. Totonchy, Jessica M. Osborn, Sara Botto, Lisa Clepper, Ashlee V. Moses
    PLoS ONE
  3. A meta-analysis of CXCL12 expression for cancer prognosis
    Authors: Harsh Samarendra, Keaton Jones, Tatjana Petrinic, Michael A Silva, Srikanth Reddy, Zahir Soonawalla et al.
    British Journal of Cancer
  4. Transcriptional and translational‐uncoupling in regulation of the CXCL12 and its receptors CXCR4, 7 in THP‐1 monocytes and macrophages
    Authors: Lu Yu, Liangli Yu, Quynhchi Pham, Thomas T. Y. Wang
    Immunity, Inflammation and Disease
  5. Platelets Fuel the Inflammasome Activation of Innate Immune Cells
    Authors: V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin
    Cell Rep, 2020-05-12;31(6):107615.
    Species: Human
    Sample Types:
    Applications: Blocking/Neutralizing
  6. Receptor-Ligand Interaction Mediates Targeting of Endothelial Colony Forming Cell-derived Exosomes to the Kidney after Ischemic Injury
    Authors: JL Viñas, M Spence, A Gutsol, W Knoll, D Burger, J Zimpelmann, DS Allan, KD Burns
    Sci Rep, 2018-11-05;8(1):16320.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Dual targeting of the chemokine receptors CXCR4 and ACKR3 with novel engineered chemokines.
    Authors: Hanes M, Salanga C, Chowdry A, Comerford I, McColl S, Kufareva I, Handel T
    J Biol Chem, 2015-07-27;290(37):22385-97.
    Species: Human
    Sample Types: Virus
    Applications: ELISA Development (Capture)
  8. COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration.
    Authors: Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Drean Y, Pakdel F
    BMC Cancer, 2014-06-06;14(0):407.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. Identification and characterization of circulating variants of CXCL12 from human plasma: effects on chemotaxis and mobilization of hematopoietic stem and progenitor cells.
    Authors: Richter R, Jochheim-Richter A, Ciuculescu F, Kollar K, Seifried E, Forssmann U, Verzijl D, Smit M, Blanchet X, von Hundelshausen P, Weber C, Forssmann W, Henschler R
    Stem Cells Dev, 2014-05-27;23(16):1959-74.
    Species: Human
    Sample Types: Plasma
    Applications: Functional Assay
  10. Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node.
    Authors: Yin X, Johns S, Kim D, Mikulski Z, Salanga C, Handel T, Macal M, Zuniga E, Fuster M
    J Immunol, 2014-02-03;192(5):2133-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  11. A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
    Authors: Ruiz E, Oeztuerk-Winder F, Ventura J
    Nat Commun, 2014-01-01;5(0):3175.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Functional Assay, Western Blot
  12. Neuroblastoma-derived TGF-beta1 modulates the chemokine receptor repertoire of human resting NK cells.
    Authors: Castriconi R, Dondero A, Bellora F, Moretta L, Castellano A, Locatelli F, Corrias M, Moretta A, Bottino C
    J Immunol, 2013-04-10;190(10):5321-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
    Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
    Eur. J. Immunol., 2008-11-01;38(11):3050-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice.
    Authors: Brzoska E, Grabowska I, Hoser G, Streminska W, Wasilewska D, Machaj EK, Pojda Z, Moraczewski J, Kawiak J
    Exp. Hematol., 2006-09-01;34(9):1262-70.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
    Authors: Carman CV, Springer TA
    J. Cell Biol., 2004-10-25;167(2):377-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Functional gene expression analysis of clonal plasma cells identifies a unique molecular profile for light chain amyloidosis.
    Authors: Abraham RS, Ballman KV, Dispenzieri A, Grill DE, Manske MK, Price-Troska TL, Paz NG, Gertz MA, Fonseca R
    Blood, 2004-09-23;105(2):794-803.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
    Authors: Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
    J. Immunol., 2004-09-15;173(6):3668-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
    Authors: Casazza S, Zappia E, Pistorio A, Gambini C
    Proc. Natl. Acad. Sci. U.S.A., 2004-07-19;101(30):11064-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003-05-01;73(5):584-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
    Authors: Cashman J, Clark-Lewis I, Eaves A, Eaves C
    Blood, 2002-02-01;99(3):792-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Chemokine mediated signalling within arteries promotes vascular smooth muscle cell recruitment
    Authors: Amber N. Stratman, Margaret C. Burns, Olivia M. Farrelly, Andrew E. Davis, Wenling Li, Van N. Pham et al.
    Communications Biology
  22. Titanium salts tested in reconstructed human skin with integrated MUTZ ‐3‐derived Langerhans cells show an irritant rather than a sensitizing potential
    Authors: Charlotte T. Rodrigues Neves, Sander W. Spiekstra, Niels P. J. de Graaf, Thomas Rustemeyer, Albert J. Feilzer, Cees J. Kleverlaan et al.
    Contact Dermatitis
  23. Evidence of lateral transmission of aggressive features between different types of breast cancer cells
    Authors: Nancy Adriana Espinoza-Sánchez, Eduardo Vadillo, Juan Carlos Balandrán, Alberto Monroy-García, Rosana Pelayo, Ezequiel M. Fuentes-Pananá
    International Journal of Oncology
  24. MUTZ-3 Langerhans Cell maturation and CXCL12 independent migration in reconstructed human gingiva
    Authors: Ilona J Kosten, Sander W Spiekstra, Tanja D de Gruijl, Susan Gibbs
    ALTEX

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