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Human CXCL11/I-TAC DuoSet ELISA

Catalog #: DY672
15 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
DY672
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human CXCL11 / I-TAC ELISA Standard Curve
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Product Details
Procedure
Citations (15)
FAQs
Supplemental Products
Reviews (1)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human CXCL11/I-TAC DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CXCL11/I-TAC. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human CXCL11 / I-TAC ELISA Standard Curve View Larger

Human CXCL11 / I-TAC ELISA Standard Curve

Product Datasheets

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Product Datasheet

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL11/I-TAC

Interferon-inducible T cell a chemoattractant (I-TAC), also known as SCYB9B, H174 and beta-R1, is a non-ELR motif-containing CXC chemokine. I-TAC shares 36% and 37% amino acid sequence homology with IP-10 and MIG, respectively. I-TAC is expressed at low levels in normal tissues, including thymus, spleen and pancreas.

Entrez Gene IDs:
6373 (Human); 56066 (Mouse)
Alternate Names:
beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human CXCL11/I-TAC DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Anti-Inflammatory and Anti-Fibrotic Effect of Immortalized Mesenchymal-Stem-Cell-Derived Conditioned Medium on Human Lung Myofibroblasts and Epithelial Cells
    Authors: E Filidou, L Kandilogia, G Tarapatzi, M Spathakis, P Steiropoul, D Mikroulis, K Arvanitidi, V Paspaliari, G Kolios
    International Journal of Molecular Sciences, 2022-04-20;23(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis
    Authors: L Kandilogia, E Filidou, I Drygiannak, G Tarapatzi, S Didaskalou, M Koffa, K Arvanitidi, G Bamias, V Valatas, V Paspaliari, G Kolios
    Stem Cells International, 2021-07-27;2021(0):9929461.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration
    Authors: HK Skovdahl, S Gopalakris, TD Svendsen, AVB Granlund, I Bakke, ZG Ginbot, S Thorsvik, A Flatberg, B Sporsheim, J Ostrop, TE Mollnes, AK Sandvik, T Bruland
    Frontiers in Pharmacology, 2021-05-13;12(0):679741.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
    Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
    Biomolecules, 2021-02-21;11(2):.
    Species: Human
    Sample Types: Plasma
  5. Tissue-specific endothelial cell heterogeneity contributes to unequal inflammatory responses
    Authors: H Gunawardan, T Romero, N Yao, S Heidt, A Mulder, DA Elashoff, NM Valenzuela
    Scientific Reports, 2021-01-21;11(1):1949.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Clinical Relevance of the Anti-inflammatory Effects of Roflumilast on Human Bronchus: Potentiation by a Long-Acting Beta-2-Agonist
    Authors: H Salvator, A Buenestado, M Brollo, E Naline, T Victoni, E Longchamp, H Tenor, S Grassin-De, P Devillier
    Frontiers in Pharmacology, 2020-12-08;11(0):598702.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. CXCL9, CXCL10, and CXCL11; biomarkers of pulmonary inflammation associated with autoimmunity in patients with collagen vascular diseases-associated interstitial lung disease and interstitial pneumonia with autoimmune features
    Authors: M Kameda, M Otsuka, H Chiba, K Kuronuma, T Hasegawa, H Takahashi, H Takahashi
    PLoS ONE, 2020-11-02;15(11):e0241719.
    Species: Human
    Sample Types: Serum
  8. Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-gamma and TNF-alpha in intestinal epithelial cells
    Authors: JA Woznicki, P Flood, M Bustamante, P Stamou, G Moloney, A Fanning, SA Zulquernai, J McCarthy, F Shanahan, S Melgar, K Nally
    Cell Death Dis, 2020-01-27;11(1):68.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. IL-27 Modulates Chemokine Production in TNF-? -Stimulated Human Oral Epithelial Cells
    Authors: Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo
    Cell. Physiol. Biochem., 2017-10-05;43(3):1198-1206.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Suction blistering the lesional skin of vitiligo patients reveals useful biomarkers of disease activity
    Authors: JP Strassner, M Rashighi, M Ahmed Refa, JM Richmond, JE Harris
    J. Am. Acad. Dermatol, 2017-03-01;0(0):.
    Species: Human
    Sample Types: Blister Fluid
  11. Effect of JAK Inhibitors on Release of CXCL9, CXCL10 and CXCL11 from Human Airway Epithelial Cells.
    Authors: Fenwick P, Macedo P, Kilty I, Barnes P, Donnelly L
    PLoS ONE, 2015-06-19;10(6):e0128757.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. CXCR3 axis in patients with primary biliary cirrhosis: a possible novel mechanism of the effect of ursodeoxycholic acid.
    Authors: Manousou P, Kolios G, Drygiannakis I, Koulentaki M, Pyrovolaki K, Voumvouraki A, Notas G, Bourikas L, Papadaki H, Kouroumalis E
    Clin Exp Immunol, 2013-04-01;172(1):9-15.
    Species: Human
    Sample Types: Serum
  13. Mouse models of rhinovirus-induced disease and exacerbation of allergic airway inflammation.
    Authors: Bartlett NW, Walton RP, Edwards MR, Aniscenko J, Caramori G, Zhu J, Glanville N, Choy KJ, Jourdan P, Burnet J, Tuthill TJ, Pedrick MS, Hurle MJ, Plumpton C, Sharp NA, Bussell JN, Swallow DM, Schwarze J, Guy B, Almond JW, Jeffery PK, Lloyd CM, Papi A, Killington RA, Rowlands DJ, Blair ED, Clarke NJ, Johnston SL
    Nat. Med., 2008-02-03;14(2):199-204.
    Species: Mouse
    Sample Types: BALF
  14. The role of IkappaB kinase 2, but not activation of NF-kappaB, in the release of CXCR3 ligands from IFN-gamma-stimulated human bronchial epithelial cells.
    Authors: Tudhope SJ, Catley MC, Fenwick PS, Russell RE, Rumsey WL, Newton R, Barnes PJ, Donnelly LE
    J. Immunol., 2007-11-01;179(9):6237-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow.
    Authors: Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH
    Am. J. Pathol., 2005-09-01;167(3):887-99.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human CXCL11/I-TAC DuoSet ELISA
By Anonymous on 04/02/2020
Sample Tested: Serum and Plasma
Human CXCL11/I-TAC DuoSet ELISA DY672