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Human Complement Component C5/C5a Biotinylated Antibody

Catalog #: BAM20371
3 Citations Datasheet
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BAM20371
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Component C5/C5a Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Component C5/C5a in ELISAs. In sandwich immunoassays, detects human Complement Component C5a by itself or in the context of Complement Component C5. In ELISAs, this antibody does not cross-react with recombinant human (rh) Complement Component C3a, rh alpha 2‑macroglobulin, recombinant mouse (rm) Complement Component C5a, or rmComplement Component C5d.
Source
Monoclonal Mouse IgG1 Clone # 295009
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Complement Component C5a
Thr678-Arg751
Accession # P01031
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human Complement Component C5/C5a Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human Complement Component C5/C5a Antibody (Catalog # MAB2037)

Standard: Recombinant Human Complement Component C5a Protein (Catalog # 2037-C5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C5/C5a

Human complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

References
  1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
  5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
Entrez Gene IDs
727 (Human); 15139 (Mouse)
Alternate Names
C5; C5a;C5b;C5D;Complement C5;CPAMD4;ECLZB; Complement Component C5/C5a

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Citations for Human Complement Component C5/C5a Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The Influence of an Elastase-Sensitive Complement C5 Variant on Lupus Nephritis and Its Flare
    Authors: CR Toy, H Song, HN Nagaraja, J Scott, J Greco, X Zhang, CY Yu, JA Tumlin, BH Rovin, LA Hebert, DJ Birmingham
    Kidney international reports, 2021-06-09;6(8):2105-2113.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  2. Establishing a Case for Anti-complement Therapy in Membranous Nephropathy
    Authors: I Ayoub, JP Shapiro, H Song, XL Zhang, S Parikh, S Almaani, S Madhavan, SV Brodsky, A Satoskar, C Bott, L Yu, M Merchant, J Klein, JM Mejia-Vile, T Nadasdy, D Birmingham, BH Rovin
    Kidney international reports, 2020-12-13;6(2):484-492.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Detection
  3. Generation of multiple fluid-phase C3b:plasma protein complexes during complement activation: possible implications in C3 glomerulopathies.
    Authors: Ramadass, Mahalaks, Ghebrehiwet, Berhane, Smith, Richard, Kew, Richard
    J Immunol, 2013-12-23;192(3):1220-30.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)

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