Human Complement Component C5/C5a Antibody Summary
Thr678-Arg751
Accession # P01031
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Complement Component C5/C5a Monoclonal Antibody (Catalog # MAB2037).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Complement Component C5a DuoSet ELISA Kit (Catalog # DY2037) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Human Complement Component C5/C5a ELISA Standard Curve. Recombinant Human Complement Component C5/C5a protein was serially diluted 2-fold and captured by Mouse Anti-Human Complement Component C5/C5a Monoclonal Antibody (Catalog # MAB2037) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Complement Component C5/C5a Monoclonal Antibody (Catalog # MAB20371) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C5/C5a
Human complement component C5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a non-signaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
- Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
- DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
- Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
- Okinaga, S. et al. (2003) Biochemistry 42:9406.
- Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
Product Datasheets
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