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Human Coagulation Factor III/Tissue Factor Antibody

Catalog #: MAB23391
2 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB23391
MAB23391-SP
Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry.
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Citations (2)
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Human Coagulation Factor III/Tissue Factor Antibody Summary

Species Reactivity
Human
Specificity
Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no
cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
Source
Monoclonal Mouse IgG1 Clone # 323519
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III
Gly34-Glu251
Accession # P13726
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Coagulation Factor III/Tissue Factor antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor Monoclonal Antibody (Catalog # MAB23391) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a type I transmembrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). TF is synthesized as a 295 amino acid (aa) precursor,  with a signal peptide (aa 1-32), an extracellular domain (aa 33-251), a transmembrane region (aa 252-274) and a cytoplasmic tail (aa 275-295) (3‑6).

References
  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
  3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
  4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
  5. Morrissey, J.H. et al. (1987) Cell 50:129.
  6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
Entrez Gene IDs
2152 (Human); 14066 (Mouse)
Alternate Names
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor

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Citations for Human Coagulation Factor III/Tissue Factor Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Tissue factor-targeted lidamycin inhibits growth and metastasis of colon carcinoma
    Authors: QING ZHANG, XIUJUN LIU, CAIHONG LI, DONGSHENG LIAO, ZHIGANG OUYANG, JUNNIAN ZHENG et al.
    Oncology Letters
  2. Chimeric antigen receptor-modified T Cells inhibit the growth and metastases of established tissue factor-positive tumors in NOG mice
    Authors: Q Zhang, H Wang, H Li, J Xu, K Tian, J Yang, Z Lu, J Zheng
    Oncotarget, 2017-02-07;8(6):9488-9499.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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