Human CNTF Biotinylated Antibody

Catalog #: BAF257 Datasheet

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BAF257

All CNTF Antibodies

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Human CNTF Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CNTF in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant rat CNTF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CNTF (R&D Systems, Catalog # 257-NT)
Ala2-Met200
Accession # P26441

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CNTF (Catalog # 257-NT)

Human CNTF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human CNTF Antibody (Catalog # MAB657)

Standard: Recombinant Human CNTF Protein (Catalog # 257-NT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name

Ciliary Neurotrophic Factor

Entrez Gene IDs

1270 (Human); 12803 (Mouse); 25707 (Rat)

Alternate Names

ciliary neurotrophic factor; CNTF; HCNTF