Human CNTF Antibody Summary
Ala2-Met200
Accession # P26441
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by CNTF and Neutralization by Human CNTF Antibody. Recombinant Human CNTF (Catalog # 257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA). The ND50 is typically 3-8 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CNTF
Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.
The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.
Product Datasheets
Citations for Human CNTF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
Authors: He W, Gong K, Smith DK, Ip NY
FEBS Lett., 2005-08-15;579(20):4317-23.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Lentiviral-mediated transfer of CNTF to schwann cells within reconstructed peripheral nerve grafts enhances adult retinal ganglion cell survival and axonal regeneration.
Authors: Leaver SG, Plant GW, Hendriks WT, Niclou SP, Verhaagen J, Harvey AR, Cui Q
Mol. Ther., 2005-06-01;11(6):906-15.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Ciliary neurotrophic factor released by corneal endothelium surviving oxidative stress ex vivo.
Authors: Koh SW
Invest. Ophthalmol. Vis. Sci., 2002-09-01;43(9):2887-96.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
Authors: He W, Gong K, Zhu G, Smith DK
FEBS Lett., 2002-03-13;514(2):214-8.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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