Human CNTF Antibody

Catalog #: MAB657 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB657
MAB657-SP

Cell Proliferation Induced by CNTF and Neutralization by Human CNTF Antibody.

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All CNTF Antibodies

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Human CNTF Antibody Summary

Species Reactivity

Human

Specificity

Detects human CNTF in ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 21809

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CTNF
Ala2-Met200
Accession # P26441.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CNTF (Catalog # 257-NT)

Human CNTF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CNTF Biotinylated Antibody (Catalog # BAF257)

Standard: Recombinant Human CNTF Protein (Catalog # 257-NT)

Neutralization

Measured by its ability to neutralize CNTF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 2-6 µg/mL in the presence of 400 ng/mL Recombinant Human CNTF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by CNTF and Neutralization by Human CNTF Antibody. Recombinant Human CNTF (Catalog # 257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657). The ND₅₀ is typically 2-6 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for human CNTF encodes a 200 amino acid polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name

Ciliary Neurotrophic Factor

Entrez Gene IDs

1270 (Human); 12803 (Mouse); 25707 (Rat)

Alternate Names

ciliary neurotrophic factor; CNTF; HCNTF