Home / Clusterin / Human Clusterin Antibody

Human Clusterin Antibody

Catalog #: MAB2937
5 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB2937
MAB2937-SP
Detection of Human Clusterin by Western Blot.
3 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Clusterin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Clusterin in direct ELISAs and Western blots. Does not cross-react with recombinant human Clusterin-like 1, recombinant mouse Clusterin, or recombinant rat Clusterin.
Source
Monoclonal Mouse IgG2B Clone # 350227
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Clusterin isoform 1
Asp23-Arg227 (beta) & Ser228-Glu449 (alpha)
Accession # NP_001822
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Clusterin (Catalog # 2937-HS), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Clusterin antibody by Western Blot. View Larger

Detection of Human Clusterin by Western Blot. Western blot shows lysates of human liver tissue and human serum. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB2937) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Clusterin Precursor at approximately 60-65 kDa and Clusterin a chain at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Clusterin antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

Clusterin in Mouse Spleen. Clusterin was detected in immersion fixed frozen sections of mouse spleen using Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB2937) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Clusterin antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Clusterin in Human Prostate Cancer Tissue. Clusterin was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB2937) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Clusterin

Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40, is a secreted multi-functional protein that was named for its ability to induce cellular clustering. It binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis (1, 2). Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development (3). Human Clusterin is synthesized as a precursor that contains two coiled coil domains, three nuclear localization signals (NLS), and one heparin binding domain (4-6). Intracellular cleavages of the precursor remove the signal peptide and generate comparably sized alpha and beta chains which are secreted as an 80 kDa N-glycosylated disulfide-linked heterodimer (7, 8). Mature human Clusterin shares 77% amino acid sequence identity with mouse and rat Clusterin. High μg/mL concentrations of Clusterin circulate predominantly as a component of high density lipoprotein particles, and these are internalized and degraded through interactions with LRP-2/Megalin (9, 10). In human, an alternately spliced 50 kDa isoform of Clusterin (nCLU) lacks the signal peptide and remains intracellular (5, 11). This molecule is neither glycosylated nor cleaved into alpha and beta chains (11). In the cytoplasm, nCLU destabilizes the actin cytoskeleton and inhibits NF kappa B activation (12, 13). Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus where it interacts with Ku70 and promotes apoptosis (5, 11). This function contrasts with the cytoprotective effect of secreted Clusterin (14). During colon cancer tumor progression there is a downregulation of the intracellular form and an upregulation of the glycosylated secreted form (11).

References
  1. Carver, J.A. et al. (2003) IUBMB Life 55:661.
  2. Shannan, B. et al. (2006) Cell Death Differ. 13:12.
  3. French, L.E. et al. (1993) J. Cell Biol. 122:1119.
  4. Kirszbaum, L. et al. (1989) EMBO J. 8:711.
  5. Leskov, K.S. et al. (2003) J. Biol. Chem. 278:11590.
  6. Pankhurst, G.J. et al. (1998) Biochemistry 37:4823.
  7. Burkey, B.F. et al. (1991) J. Lipid. Res. 32:1039.
  8. de Silva, H.V. et al. (1990) J. Biol. Chem. 265:14292.
  9. Jenne, D.E. et al. (1991) J. Biol. Chem. 266:11030.
  10. Kounnas, M.Z. et al. (1995) J. Biol. Chem. 270:13070.
  11. Pucci, S. et al. (2004) Oncogene 23:2298.
  12. Moretti, R. M. et al. (2007) Cancer Res. 67:10325.
  13. Santilli, G. et al. (2003) J. Biol. Chem. 278:38214.
  14. Trougakos, I.P. et al. (2004) Cancer Res. 64:1834.
Entrez Gene IDs
1191 (Human); 12759 (Mouse)
Alternate Names
40; 40, sulfated glycoprotein 2; Aging-associated gene 4 protein; aging-associated protein 4; APOJ; apo-J; Apolipoprotein J; CLI; CLIclusterin (complement lysis inhibitor, SP-40; CLU; Clusterin; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40; Ku70-binding protein 1; KUB1SGP2; MGC24903; NA1/NA2; SGP-2; SP-40; sulfated glycoprotein 2; Testosterone-repressed prostate message 2; testosterone-repressed prostate message 2, apolipoprotein J); TRPM-2; TRPM-2TRPM2

Product Datasheets

You must select a language.

x
Product Datasheet

Citations for Human Clusterin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.
    Authors: Kinchen J, Chen HH, Parikh K et al.
    Cell
  2. Divergent single cell transcriptome and epigenome alterations in ALS and FTD patients with C9orf72 mutation
    Authors: Li J, Jaiswal MK, Chien JF et al.
    Nat Commun
  3. Divergent single cell transcriptome and epigenome alterations in ALS and FTD patients with C9orf72 mutation
    Authors: Li J, Jaiswal MK, Chien JF et al.
    Nat Commun
  4. Low-dose brain irradiation normalizes TSPO and CLUSTERIN levels and promotes the non-amyloidogenic pathway in pre-symptomatic TgF344-AD rats
    Authors: K Ceyzériat, T Zilli, P Millet, N Koutsouvel, G Dipasquale, C Fossey, T Cailly, F Fabis, GB Frisoni, V Garibotto, BB Tournier
    Journal of Neuroinflammation, 2022-12-22;19(1):311.
    Species: Rat
    Sample Types: Protein
    Applications: Western Blot
  5. Brain clusterin protein isoforms and mitochondrial localization
    Authors: Sarah K Herring, Hee-Jung Moon, Punam Rawal, Anindit Chhibber, Liqin Zhao
    eLife

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Clusterin Antibody

There are currently no reviews for this product. Be the first to review Human Clusterin Antibody and earn rewards!

Have you used Human Clusterin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review