Human cIAP-1/HIAP-2 Antibody Summary
His2-Ser618
Accession # Q13490
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human cIAP-1/HIAP-2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with full length human cIAP-1, or transfected with full length human cIAP-2, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human cIAP-1/HIAP-2 Monoclonal Antibody (Catalog # MAB818) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for cIAP-1/HIAP-2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
View Larger
Detection of Human cIAP‑1/HIAP‑2 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB818). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
View Larger
Western Blot Shows Human cIAP-1/HIAP-2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human cIAP-1/HIAP-2 Monoclonal Antibody (Catalog # MAB818) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for cIAP-1/HIAP-2 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Specificity of Human cIAP‑1/HIAP‑2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP‑1/HIAP‑2 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. Mouse Anti-Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB818) was used at 5 µg/mL. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP-1/HIAP-2
cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.
- Roy, N. et al. (1997) EMBO J. 23:6914.
- Deveraux, Q. et al. (1997) Nature 388:300.
- Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
- Herrera, B. et al. (2002) FEBS Letters 520:93.
Product Datasheets
Citation for Human cIAP-1/HIAP-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
A novel function of cIAP1 as a mediator of CHIP-driven eIF4E regulation
Authors: TW Seo, JS Lee, YN Choi, DH Jeong, SK Lee, SJ Yoo
Sci Rep, 2017-08-29;7(1):9816.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human cIAP-1/HIAP-2 Antibody
There are currently no reviews for this product. Be the first to review Human cIAP-1/HIAP-2 Antibody and earn rewards!
Have you used Human cIAP-1/HIAP-2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
