Human CD8 alpha PerCP-conjugated Antibody

Catalog #: FAB1509C Datasheet

Catalog #	Availability	Size / Price	Qty
FAB1509C-100
FAB1509C-025

Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry.

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Human CD8 alpha PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse CD8 alpha is observed.

Source

Monoclonal Mouse IgG_2B Clone # 37006

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD8 alpha
Ser22-Val198
Accession # NP_741969

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8a PerCP-conjugated Monoclonal Antibody (Catalog # FAB1509C) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041C) and Mouse Anti-Human CD3e CFS-conjugated Monoclonal Antibody (Catalog # FAB100F). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Alternate Names

CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2