Human CD8 alpha Antibody

Catalog #: MAB3802 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB3802-100
MAB3802-SP

Detection of CD8 in Human Blood Lymphocytes by Flow Cytometry.

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Human CD8 alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD8 alpha in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # RPA-T8

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CD8 alpha
Accession # NP_741696

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD8 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8 Monoclonal Antibody (Catalog # MAB3802) or (B) Mouse IgG1 Isotype Control (MAB002), followed by PE-conjugated anti-Mouse IgG secondary antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (FAB100A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Alternate Names

CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2