Human CD44 v6 PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD44 v6 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD44 v6 PE-conjugated Monoclonal Antibody (Catalog # FAB3660P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD44
CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1-3). The standard or hematopoietic form, CD44H, does not include the variable segments (1-3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83% and 79% aa sequence identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).
- Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
- Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
- Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
- Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
- Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
- Nakamura, H. et al. (2004) Cancer Res. 64:876.
- Murakami, D. et al. (2003) Oncogene 22:1511.
- Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
- Fox, S.B. et al. (1994) Cancer Res. 54:4539.
Product Datasheets
Citations for Human CD44 v6 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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HMSCs exosome‐derived miR‐199a‐5p attenuates sulfur mustard‐associated oxidative stress via the CAV1/NRF2 signalling pathway
Authors: Chuchu Gong, Zhengyan Gu, Xinkang Zhang, Qingqiang Xu, Guanchao Mao, Zhipeng Pei et al.
Journal of Cellular and Molecular Medicine
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CAR-NK Cells Targeting HER1 (EGFR) Show Efficient Anti-Tumor Activity against Head and Neck Squamous Cell Carcinoma (HNSCC)
Authors: Nowak, J;Bentele, M;Kutle, I;Zimmermann, K;Lühmann, JL;Steinemann, D;Kloess, S;Koehl, U;Ro beta berg, W;Ahmed, A;Schaudien, D;Neubert, L;Kamp, JC;Kuehnel, MP;Warnecke, A;Schambach, A;Morgan, M;
Cancers
Species: Human
Sample Types: Transduced Whole Cells, Whole Cells
Applications: Flow Cytometry -
NK Cells Lose Their Cytotoxicity Function against Cancer Stem Cell-Rich Radiotherapy-Resistant Breast Cancer Cell Populations
Authors: H Jin, HJ Kim
International Journal of Molecular Sciences, 2021-09-06;22(17):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
LncRNA AFAP1-AS1/miR-27b-3p/VEGF-C axis modulates stemness characteristics in cervical cancer cells
Authors: Meng Xia, Li-Jun Duan, Bi-Nan Lu, Yu-Zhou Pang, Zong-Ran Pang
Chinese Medical Journal
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Baseline CD44v6-positive circulating tumor cells to predict first-line treatment failure in patients with metastatic colorectal cancer
Authors: C Nicolazzo, F Loreni, S Caponnetto, V Magri, AR Vestri, R Zamarchi, A Gradilone, A Facchinett, E Rossi, E Cortesi, P Gazzaniga
Oncotarget, 2020-11-10;11(45):4115-4122.
Species: Human
Sample Types: Whole Blood
Applications: Bioassay -
Clinicopathological significance and prognostic implication of CD44 and its splice variants (v3 and v6) in colorectal cancer
Authors: Bing Yan, Ying Mu, Meiqi Cui, Long Liu
Translational Cancer Research
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Musashi2 contributes to the maintenance of CD44v6+ liver cancer stem cells via notch1 signaling pathway
Authors: Xiju Wang, Ronghua Wang, Shuya Bai, Si Xiong, Yawen Li, Man Liu et al.
Journal of Experimental & Clinical Cancer Research
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Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance
Authors: KA Whelan, PM Chandramou, K Tanaka, M Natsuizaka, M Guha, S Srinivasan, DS Darling, Y Kita, S Natsugoe, JD Winkler, AJ Klein-Szan, RK Amaravadi, NG Avadhani, AK Rustgi, H Nakagawa
Oncogene, 2017-04-17;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Coexpression of EpCAM, CD44 variant isoforms and claudin-7 in anaplastic thyroid carcinoma.
Authors: Okada, Toshihir, Nakamura, Teruo, Watanabe, Takayuki, Onoda, Naoyoshi, Ashida, Atsuko, Okuyama, Ryuhei, Ito, Ken-ichi
PLoS ONE, 2014-04-11;9(4):e94487.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Hypoxia regulates CD44 and its variant isoforms through HIF-1alpha in triple negative breast cancer.
Authors: Krishnamachary B, Penet M, Nimmagadda S, Mironchik Y, Raman V, Solaiyappan M, Semenza G, Pomper M, Bhujwalla Z
PLoS ONE, 2012-08-28;7(8):e44078.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Preferential migration of T regulatory cells induced by IL-16.
Authors: McFadden C, Morgan R, Rahangdale S, Green D, Yamasaki H, Center D, Cruikshank W
J. Immunol., 2007-11-15;179(10):6439-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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