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Human CD40 Ligand/TNFSF5 Biotinylated Antibody

Catalog #: BAF617
1 Citation Datasheet
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BAF617
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD40 Ligand/TNFSF5 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD40 Ligand in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CD40 Ligand
Glu108-Leu261
Accession # P29965
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 (Catalog # 6245-CL)
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD40 Ligand/TNFSF5

CD40 Ligand is a type II transmembrane glycoprotein belonging to the TNF family (TNFSF5). It is expressed predominantly on activated CD4+ T lymphocytes and also on other hematopoietic cells. CD40 L binds to CD40 (TNFRSF5) on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Engagement of CD40 by CD40 Ligand promotes B cell and dendritic cell maturation and activation.

Entrez Gene IDs
959 (Human); 21947 (Mouse); 84349 (Rat)
Alternate Names
CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

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Citation for Human CD40 Ligand/TNFSF5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Soluble CD40 ligand as a biomarker for storage-related preanalytic variations of human serum.
    Authors: Lengelle J, Panopoulos E, Betsou F
    Cytokine, 2008-10-11;44(2):275-82.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot

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