Human CD40 Ligand/TNFSF5 Antibody Summary
Glu108-Leu261
Accession # P29965
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CD40 Ligand/TNFSF5 by Western Blot. Western blot shows conditioned media (CM) from Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD40 Ligand/TNFSF5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Detection of Human CD40 Ligand/TNFSF5 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cells, Human Platelets and Th1 CD4+. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD40 Ligand/TNFSF5 at approximately 17 and 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD40 Ligand/TNFSF5
CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its murine counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.
- Armitage, R.J. et al. (1992) Nature 357:80.
- Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
- Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543.
- Fanslow, W.C. et al. (1994) Seminars in Immunology 6:267.
- Kooten, C.V. and J. Banchereau (2000) J. Leukoc. Biol. 67:2.
Product Datasheets
Citations for Human CD40 Ligand/TNFSF5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Intradermal but not intramuscular modified vaccinia Ankara immunizations protect against intravaginal tier2 simian-human immunodeficiency virus challenges in female macaques
Authors: Bollimpelli, VS;Reddy, PBJ;Gangadhara, S;Charles, TP;Burton, SL;Tharp, GK;Styles, TM;Labranche, CC;Smith, JC;Upadhyay, AA;Sahoo, A;Legere, T;Shiferaw, A;Velu, V;Yu, T;Tomai, M;Vasilakos, J;Kasturi, SP;Shaw, GM;Montefiori, D;Bosinger, SE;Kozlowski, PA;Pulendran, B;Derdeyn, CA;Hunter, E;Amara, RR;
Nature communications
Species: Human
Sample Types: Cell Lysates
Applications: Flow Cytometry -
V2 hotspot optimized MVA vaccine expressing stabilized HIV-1 Clade C envelope Gp140 delays acquisition of heterologous Clade C Tier 2 challenges in Mamu-A*01 negative Rhesus Macaques
Authors: Tiffany M. Styles, Sailaja Gangadhara, Pradeep B. J. Reddy, Anusmita Sahoo, Ayalensh Shiferaw, Sarah Welbourn et al.
Frontiers in Immunology
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Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
Redox Biology, 2021-12-05;48(0):102205.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Dot Blot -
Systemic immunity upon local oncolytic virotherapy armed with immunostimulatory genes may be supported by tumor-derived exosomes
Authors: Alireza Labani-Motlagh, Sedigheh Naseri, Jessica Wenthe, Emma Eriksson, Angelica Loskog
Molecular Therapy - Oncolytics
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Human Immunodeficiency Virus C.1086 Envelope gp140 Protein Boosts following DNA/Modified Vaccinia Virus Ankara Vaccination Fail To Enhance Heterologous Anti-V1V2 Antibody Response and Protection against Clade C Simian-Human Immunodeficiency Virus Challenge
Authors: Tiffany M. Styles, Sailaja Gangadhara, Pradeep B. J. Reddy, Sakeenah Hicks, Celia C. LaBranche, David C. Montefiori et al.
Journal of Virology
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CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals.
Authors: Liu J, Yu Q, Stone GW, Yue FY, Ngai N, Jones RB, Kornbluth RS, Ostrowski MA
Vaccine, 2008-06-02;26(32):4062-72.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Authors: Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
J. Immunol., 2006-05-15;176(10):5735-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
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