Human CD4 PE-conjugated Antibody

Catalog #: FAB114341P Datasheet

Catalog #	Availability	Size / Price	Qty
FAB114341P-100

Detection of CD4 in PBMC lymphocytes by Flow Cytometry.

2 Images

All CD4 Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD4 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD4 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1068606

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human CD4
Gln26-Lys463
Accession # P08575

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

PBMC lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD4 in PBMC lymphocytes by Flow Cytometry. PBMC lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB114341P) or (B) Mouse IgG_2B Phycoerythrin Isotype Control (Catalog # IC0041P) followed by. View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of CD4 in Human peripheral blood lymphocytes by Flow Cytometry Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB114341P) and Mouse Anti-Human CD4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB37912R). This demonstrates non-competing epitopes of the two clones, which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) precursor protein with a 25 aa signal peptide, a 371 aa extracellular region containing four immunoglobulin homology domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References

Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4