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Human CD4 mFluor™ Violet 500-Conjugated Antibody

Catalog #: FAB3791MFV500 Datasheet
Catalog # Availability Size / Price Qty
FAB3791MFV500-100
Detection of CD4 in PBMCs by Flow Cytometry.
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Human CD4 mFluor™ Violet 500-Conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD4 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 11830
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant Human CD4.
Extracellular Domain
Accession # P01730
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
mFluor Violet 500 SE (Excitation= 410 nm, Emission= 501 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
PBMCs with CD3 costain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of CD4 in PBMCs by Flow Cytometry. PBMCs were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD4 mFluor™ Violet 500-Conjugated Monoclonal Antibody (Catalog # FAB3791MFV500) or (B) unstained cells. View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Product Specific Notices

mFluorTM is a trademark of AAT Bioquest, Inc.

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