Human CD4 MAb (Clone 2759D)

Catalog #: MAB37914 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB37914-100
MAB37914-SP

Detection of CD4 in Human PBMC Lymphocytes by Flow Cytometry.

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Human CD4 MAb (Clone 2759D) Summary

Species Reactivity

Human

Specificity

Detects human CD4 in direct ELISAs

Source

Recombinant Monoclonal Rabbit IgG Clone # 2759D

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CD4
Lys26-Trp390
Accession # P01730.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD4 in Human PBMC Lymphocytes by Flow Cytometry. Human PBMC were stained with (A) Rabbit Anti-Human CD4 Monoclonal Antibody (Catalog # MAB37914) or (B) Rabbit IgG Control (MAB1050), followed by PE-conjugated anti-Rabbit IgG secondary antibody (F0110) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (FAB100A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). Mature human CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (2). Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4⁺ or CD8⁺ mature T cells (3). CD4⁺ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4⁺ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (4). CD4 is reexpressed on circulating CD8⁺ T cells upon activation and contributes to their cytotoxic effector activity (5). In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain (6-9). Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse (6). CD4 binds directly to MHC class II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction (1, 11). Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck (12). CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1 (7, 13-15).

References

Vignali, D.A.A. (2010) J. Immunol. 184:5933.
Maddon, P.J. et al. (1985) Cell 42:93.
Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11.
Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20.
Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794.
Crocker, P.R. et al. (1987) J. Exp. Med. 166:613.
Biswas, P. et al. (2003) Blood 101:4452.
Bernstein, H.B. et al. (2006) J. Immunol. 177:3669.
Funke, I. et al. (1987) J. Exp. Med. 165:1230.
Doyle, C. and J.L. Strominger (1987) Nature 330:256.
Huppa, J.B. et al. (2010) Nature 463:963.
Fragoso, R. et al. (2003) J. Immunol. 170:913.
Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109.
Klatzmann, D. et al. (1984) Nature 312:767.
Dagleish, A.G. et al. (1984) Nature 312:763.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4