Human CD4 Antibody

Catalog #: MAB114341 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB114341-100
MAB114341-SP

Detection of CD4 in PBMC lymphocytes stained with CD4 RPA-T4 by Flow Cytometry.

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Human CD4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD4 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1068606

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human CD4
Gln26-Lys463
Accession # P08575

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

PBMC Lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD4 in PBMC lymphocytes stained with CD4 RPA-T4 by Flow Cytometry. PBMC lymphocytes were stained with CD4 RPA-T4 (MAB37912). Cells were washed and stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog #MAB114341) or (B) Mouse IgG2B Isotype Control (MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC conjugated Monoclonal Antibody (FAB100A). This demonstrates non-competing epitopes of MAB114341 and MAB37912 which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CD4 in Human peripheral blood lymphocytes by Flow Cytometry Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB114341) which was PE-conjugated (FAB114341P), and Mouse Anti-Human CD4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (FAB37912R). This demonstrates non-competing epitopes of the two clones, which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) precursor protein with a 25 aa signal peptide, a 371 aa extracellular region containing four immunoglobulin homology domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References

Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4