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Human CD3 epsilon PerCP-conjugated Antibody

Catalog #: FAB100C
1 Citation Datasheet
Clone UCHT1 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB100C-100
FAB100C-025
Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry.
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Human CD3 epsilon PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Source
Monoclonal Mouse IgG1 Clone # UCHT1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human thymocytes (1)
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD3e antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD3e PerCP-conjugated Monoclonal Antibody (Catalog # FAB100C) or (B) Mouse IgG1PerCP Isotype Control (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References
  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Entrez Gene IDs
916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names
CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Citation for Human CD3 epsilon PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia.
    Authors: Parrish C, Scott G, Migneco G, Scott K, Steele L, Ilett E, West E, Hall K, Selby P, Buchanan D, Varghese A, Cragg M, Coffey M, Hillmen P, Melcher A, Errington-Mais F
    Leukemia, 2015-03-27;29(9):1799-810.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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