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Human CD3 epsilon F(ab')2 Antibody

Catalog #: MAB100R-FAB2 Datasheet
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB100R-FAB2-100
MAB100R-FAB2-SP
MAB100R-FAB2-500
MAB100R-FAB2-01M
Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry with anti-Mouse Secondary Antibody.
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Human CD3 epsilon F(ab')2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD3 epsilon
Source
Recombinant Monoclonal Mouse IgG1 Clone # UCHT1R-FAB2-TEV/His
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human thymocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
PBMC lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry with anti-Mouse Secondary Antibody. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD3e FAB2 Recombinant Monoclonal Antibody (Catalog # MAB100R-FAB2) or (B) Mouse IgG1 isotype control antibody (MAB002), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P). Staining was performed using our staining membrane proteins protocol.

Flow Cytometry View Larger

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry with anti-His antibody. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD3e FAB2 Recombinant Monoclonal Antibody (Catalog # MAB100R-FAB2) or (B) Mouse IgG1 isotype control antibody (MAB002), followed by PE-conjugated Anti-His Antibody (IC050P) and Mouse anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A). Staining was performed using our staining membrane proteins protocol.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References
  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Entrez Gene IDs
916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names
CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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