Human CD34 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF7227V Datasheet

Catalog #	Availability	Size / Price	Qty
AF7227V-100UG

All CD34 Antibodies

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Human CD34 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD34 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD34 and recombinant rat CD34 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD34
Ser32-Thr290
Accession # P28906

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD34

CD34 is a 105-115 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, stem cells and renal mesangial cells. Mature human CD34 is a 354 amino acid (aa) type I transmembrane protein (aa 32-385). It contains a 259 aa extracellular region (aa 35-287) with utilized N- and O-linked glycosylation sites, and a 74 aa cytoplasmic domain that may undergo Tyr phosphorylation. There is one splice variant that shows a four aa substitution for aa 325-385. Human CD34 can undergo membrane cleavage by bacterial proteases to generate 30-40 kDa soluble fragments. And notably, desialylated CD34 shows a 40 kDa increase in MW (to 150 kDa) when run in SDS-PAGE. Over aa 32-290, human CD34 shares 56% aa identity with mouse CD34.

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Alternate Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1