Human CD31/PECAM-1 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.62 | 4.7 | 9.96 | 1.71 | 5 | 10.6 |
| Standard Deviation | 0.065 | 0.117 | 0.372 | 0.104 | 0.252 | 0.411 |
| CV% | 4 | 2.5 | 3.7 | 6.1 | 5 | 3.9 |
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 2.01 | 5.86 | 12.3 | 1.9 | 5.71 | 12 |
| Standard Deviation | 0.07 | 0.182 | 0.403 | 0.163 | 0.363 | 0.677 |
| CV% | 3.5 | 3.1 | 3.3 | 8.6 | 6.4 | 5.6 |
Recovery
The recovery of human CD31 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 96-112 |
| EDTA Plasma (n=4) | 100 | 97-106 |
| Heparin Plasma (n=4) | 101 | 94-108 |
| Serum (n=4) | 104 | 100-107 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CD31/PECAM-1
CD31/PECAM-1 is a heavily glycosylated transmembrane homophilic adhesion protein that is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and NK cells. It is required for the transepithelial migration of leukocytes. CD31 signaling increases the activation threshold of T cells and inhibits dendritic cell development. The extracellular domain of CD31 is released during endothelial cell apoptosis. This fragment circulates in the serum of patients suffering from myocardial infarction, acute ischaemic stroke, and multiple sclerosis, conditions that involve tissue damage and endothelial cell apoptosis.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human CD31/PECAM-1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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IL-11 system participates in pulmonary artery remodeling and hypertension in pulmonary fibrosis
Authors: J Milara, I Roger, P Montero, E Artigues, J Escrivá, J Cortijo
Respiratory Research, 2022-11-15;23(1):313.
Species: Human
Sample Types: Serum
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A preliminary study of pamidronic acid downregulation of angiogenic factors IGF-1/PECAM-1 expression in circulating level in bone metastatic breast cancer patients
Authors: Zeng Wang
Onco Targets Ther, 2016-05-26;9(0):3147-52.
Species: Human
Sample Types: Serum
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