Human CD31/PECAM-1 Fluorescein-conjugated Antibody

Catalog #: FAB3567F
3 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
FAB3567F-100
FAB3567F-025

Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry.

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All CD31/PECAM-1 Antibodies

Product Details

Citations (3)

FAQs

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD31/PECAM-1 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) E‑Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 9G11

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CD31/PECAM-1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3567F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Alternate Names

adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human CD31/PECAM-1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
Stem Cell Res Ther, 2019-11-04;10(1):316.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells.
Authors: White M, Rufaihah A, Liu L, Ghebremariam Y, Ivey K, Cooke J, Srivastava D
Stem Cells, 2013-01-01;31(1):92-103.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells.
Authors: Gutierrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montano LF, Lopez-Marure R
Atherosclerosis, 2006-03-30;190(1):90-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry