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Human CD2 Antibody

Catalog #: MAB18561 Datasheet
Catalog # Availability Size / Price Qty
MAB18561
MAB18561-SP
CD2 in Human PBMCs.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human CD2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) CD2 or rmCD2F-10 is observed.
Source
Monoclonal Mouse IgG1 Clone # 299812
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD2
Lys25-Asp209
Accession # P06729
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human whole blood lymphocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD2 in Human PBMCs. CD2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB18561) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD2

CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T, NK, B, and some antigen presenting cells. Human CD2 binds human CD58 on antigen-presenting cells to induce co-stimulating signals in T cells.

Entrez Gene IDs
914 (Human); 12481 (Mouse); 102120798 (Cynomolgus Monkey)
Alternate Names
CD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

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